S100B

Gene Symbol S100B
Entrez Gene 6285
Alt Symbol NEF, S100, S100-B, S100beta
Species Human
Gene Type protein-coding
Description S100 calcium binding protein B
Other Description S-100 calcium-binding protein, beta chain|S-100 protein subunit beta|S100 calcium-binding protein, beta (neural)|protein S100-B
Swissprots P04271 D3DSN6
Accessions AAA60367 EAX09267 EAX09268 EAX09269 EAX09270 P04271 AA310307 BC001766 AAH01766 BC041935 CR542123 CAG46920 DQ892491 ABM83417 EB386131 EU176695 ABW03496 NM_006272 NP_006263
Function Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. {ECO:0000269|PubMed:20351179, ECO:0000269|PubMed:22399290}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:9925766}. Nucleus {ECO:0000269|PubMed:9925766}.
Tissue Specificity Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

S100B (D10G6) Rabbit mAb - 9550 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10G6
Western blot analysis of extracts from mouse brain, C2C12 cells, rat brain, and C6 cells using S100B (D10G6) Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower).

S-100 α/β chain (6G1) - sc-52206 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
S-100 α/β chain (6G1): sc-52206. Western blot analysis of human recombinant S-100 β.

S-100 β chain (9A11B9) - sc-81709 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
S-100 β chain (9A11B9): sc-81709. Western blot analysis of full-length human recombinant S-100 β chain protein.

S-100 β chain (H-56) - sc-28533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
S-100 β chain (H-56): sc-28533. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

S-100 β chain (C-20) - sc-7851 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

S-100 β chain (N-15) - sc-7852 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
S-100 β chain (N-15): sc-7852. Western blot analysis of human recombinant S-100 β.

S-100 β chain (19) - sc-136061 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
S-100 β chain (19): sc-136061. Western blot analysis of S-100 β chain expression in mouse cerebellum tissue extract.

Anti-S100 beta antibody - ab14688 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-S100 beta antibody - ab189418 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin fixed paraffin embedded human cerebellum labeling S100-beta with ab189418 at 10µg/ml.

Anti-S100 beta antibody - ab110979 from Abcam

Prices $372.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-S100 beta antibody - ab90559 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-S100 beta antibody (ab90559) at 5 µg/ml + Tagged recombinant Human S100 beta protein at 0.2 µg

Anti-S100 beta antibody - ab166594 from Abcam

Prices $370.00
Sizes 150 µg
Host Chicken
Clonality Polyclonal

Anti-S100 beta antibody - ab166964 from Abcam

Prices $0.00
Sizes 500 µl
Host Sheep
Clonality Polyclonal

Anti-S100 beta antibody [6G1] - ab20327 from Abcam

Prices $370.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone 6G1

Anti-S100 beta antibody [8B10] - ab20645 from Abcam

Prices $370.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone 8B10

Anti-S100 beta antibody [9A11B9] - ab109655 from Abcam

Host Mouse
Clonality Monoclonal
Clone 9A11B9
Anti-S100 beta antibody [9A11B9] (ab109655) at 1/500 dilution + Full-lenght S100 beta-GST recombinant protein

Anti-S100 beta antibody (Biotin) - ab105371 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-S100 beta antibody (Biotin) - ab166597 from Abcam

Prices $370.00
Sizes 150 µg
Host Chicken
Clonality Polyclonal

Anti-S100 beta antibody [EP1576Y] - ab52642 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1576Y
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A-375 (Human malignant melanoma cell line) cells labeling S100 beta with purified ab52642 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) ab150077 secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue). The negative control is as follows;ab52642 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution. 

Anti-S100 beta antibody [EP1576Y] (Alexa Fluor® 488) - ab196442 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1576Y
Overlay histogram showing A375 cells stained with ab196442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196442, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-S100 beta antibody [EP1576Y] (Alexa Fluor® 647) - ab196175 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1576Y
ab196175 staining S100 beta in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in A375 cells fixed with 4% formaldehyde (10 min).

Anti-S100 beta antibody [SH-B1] - ab11178 from Abcam

Prices $388.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone SH-B1

Anti-S100 beta antibody [SH-B1], prediluted - ab51899 from Abcam

Prices $370.00
Sizes 5 ml
Host Mouse
Clonality Monoclonal
Clone SH-B1

Anti-S100 beta antibody [SH-B4] - ab11179 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone SH-B4
Immunocytochemistry/Immunoflyorescence analysis of schwann cell (SC) -like cells induced from rat adipose-derived stem cells (ADSC) labelling S100 beta with ab11179. Nuclei are labeled with DAPI (blue). Cells were fixed in 4% paraformaldehyde, blocked with normal goat serum, and incubated with the primary antibody (1:500) overnight at 4°C. Followed by incubation with secondary antibody at room temperature for 1 hour.

Anti-S100 beta antibody - Astrocyte Marker - ab41548 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab41548 staining S100 beta in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.At this dilution factor, there is very good delineation of astrocyte processes.See Abreview
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