Anti-S100 beta antibody [EP1576Y]

Name	Anti-S100 beta antibody [EP1576Y]
Supplier	Abcam
Catalog	ab52642
Prices	$403.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EP1576Y
Applications	ICC/IF ICC/IF WB IP IHC-P
Species Reactivities	Mouse, Rat, Goat, Human, Zebrafish, Monkey
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human S100 beta aa 50 to the C-terminus (C terminal)
Description	Rabbit Monoclonal
Gene	S100B
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A-375 (Human malignant melanoma cell line) cells labeling S100 beta with purified ab52642 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) ab150077 secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue). The negative control is as follows;ab52642 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

Immunohistochemical analysis of paraffin embedded Human cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.Negative control: Using PBS instead of primary antibody.

Immunohistochemical analysis of paraffin embedded Rat cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.Negative control: Using PBS instead of primary antibody.

Immunohistochemical analysis of paraffin embedded Mouse cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.Negative control: Using PBS instead of primary antibody.

Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/5000 dilution (purified) + B16F0 (Mouse melanoma cell line) at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/10000 dilution (purified)Lane 1 : Mouse spinal cordLane 2 : Rat brainLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/5000 dilution (purified) + A-375 (Human malignant melanoma cell line) at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

S100 beta was immunoprecipitated from Human fetal brain with purified ab52642 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52642 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution.Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/1000 dilution (unpurified) + A375 cell lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled at 1/2000 dilution

Unpurified ab52642 staining S100 beta in Human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/200 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Unpurified ab52642 at 1/500 staining human melanoma tissue sections by IHC-P. The tissue was formladehyde fixed and a heat mediated antigen retrieval step was performed, before being blocked and incubated with the antibody for 1 hour. An HRP conjugated goat anti-rabbit antibody was used as the secondary.See Abreview

Immunohistochemical analysis of embryonic mouse brain tissue, staining S100 beta with unpurified ab52642.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

Distribution and development of peripheral glial cells in the human fetal - Distribution and development of peripheral glial cells in the human fetal

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S100A9 knockout decreases the memory impairment and neuropathology in crossbreed - S100A9 knockout decreases the memory impairment and neuropathology in crossbreed

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Regulation of S100B in white adipose tissue by obesity in mice. - Regulation of S100B in white adipose tissue by obesity in mice.

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Biochemical assessment of precuneus and posterior cingulate gyrus in the context - Biochemical assessment of precuneus and posterior cingulate gyrus in the context

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3-D imaging and illustration of mouse intestinal neurovascular complex. - 3-D imaging and illustration of mouse intestinal neurovascular complex.

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