Anti-S100 beta antibody - Astrocyte Marker
| Name | Anti-S100 beta antibody - Astrocyte Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab41548 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Pig, Rabbit, Bovine, Human, Hamster |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Rat S100 beta |
| Description | Rabbit Polyclonal |
| Gene | S100B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab41548 staining S100 beta in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.At this dilution factor, there is very good delineation of astrocyte processes.See Abreview
All lanes : Anti-S100 beta antibody - Astrocyte Marker (ab41548) at 1 µg/mlLane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Brain (Rat) Tissue LysateLane 3 : Spinal Cord (Mouse) Tissue Lysate Lane 4 : Spinal Cord (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ab41548 staining S100 beta in Zebrafish body, showing T/S of spinal cord region by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.See Abreview
ICC/If image of S100 beta staining using P3 rat glial cell culture using ab41548 (1:200). The cells were fixed with 4% PFA in 0.1M PBS. Primary antibody was detected using Donkey anti-rabbit Alexa Fluor 488(1:1000)See Abreview
ab41548 staining S100 beta in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.See Abreview
Product References
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