CTCF

Gene Symbol CTCF
Entrez Gene 10664
Alt Symbol MRD21
Species Human
Gene Type protein-coding
Description CCCTC-binding factor (zinc finger protein)
Other Description 11 zinc finger transcriptional repressor|11-zinc finger protein|CTCFL paralog|transcriptional repressor CTCF
Swissprots Q59EL8 Q53XI7 B5MC38 P49711
Accessions AAF31318 CAH05607 EAW83142 EAW83143 EAW83144 EAW83145 EAW83146 P49711 AA814313 AB209793 BAD93030 AI753629 AK093395 BAG52706 AK314804 BC014267 AAH14267 BT009915 AAP88917 DB229066 DQ895834 ABM86760 EU176369 ABW03820 U25435 AAB07788 XM_005255775 XP_005255832 NM_001191022 NP_001177951 NM_006565 NP_006556
Function Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over
Subcellular Location Nucleus, nucleoplasm. Chromosome. Chromosome, centromere. Note=May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.
Tissue Specificity Ubiquitous. Absent in primary spermatocytes. {ECO:0000269|PubMed:9591631}.

CTCF (D1A7) XP ® Rabbit mAb - 3417 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1A7
Western blot analysis of extracts from various cell lines using CTCF (D1A7) XP ® Rabbit mAb.

CTCF (D31H2) XP ® Rabbit mAb - 3418 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D31H2
Western blot analysis of extracts from various cell lines using CTCF (D31H2) XP ® Rabbit mAb.

CTCF Antibody - 2899 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using CTCF Antibody.

CTCF (B-5) - sc-271514 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CTCF (B-5): sc-271514. Western blot analysis of CTCF expression in Jurkat nuclear extract.

CTCF (C-9) - sc-398149 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CTCF (C-9): sc-398149. Western blot analysis of CTCF expression in K-562 (A), Jurkat (B), CCRF-CEM (C) and HeLa (D) nuclear extracts and MCF7 (E) and HeLa (F) whole cell lysates.

CTCF (H-280) - sc-28198 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CTCF (H-280): sc-28198. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.

CTCF (G-8) - sc-271474 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CTCF (G-8): sc-271474. Western blot analysis of CTCF expression in Jurkat nuclear extract.

CTCF (N-17) - sc-5916 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CTCF (N-17): sc-5916. Western blot analysis of CTCF expression in Jurkat (A) and K-562 (B) nuclear extracts.

CTCF (E-14) - sc-15913 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CTCF (E-14): sc-15913. Western blot analysis of CTCF expression in non-transfected: sc-117752 (A) and mouse CTCF transfected: sc-119494 (B) 293T whole cell lysates.

CTCF (C-20) - sc-15914 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CTCF siRNA (h): sc-35124. Western blot analysis of CTCF expression in non-transfected control (A) and CTCF siRNA transfected (B) HeLa cells. Blot probed with CTCF (C-20): sc-15914. α-actinin (H-2): sc-17829 used as specificity and loading control.

CTCF (48) - sc-136284 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
TCF (48): sc-136284. Immunofluorescence staining of A-431 cells showing nuclear localization.

Anti-CTCF antibody - ab84372 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84372 at 1/250, staining human CTCF in a section of human ovarian carcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-CTCF antibody [EPR7313] - ab128909 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7313
All lanes : Anti-CTCF antibody [EPR7313] (ab128909) at 1/1000 dilutionLane 1 : U937 cell lysateLane 2 : HeLa cell lysateLane 3 : MDA-MB-435 cell lysateLane 4 : 293T cell lysateLane 5 : Caco-2 cell lysateLane 6 : RAW 264.7 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-CTCF antibody [EPR7314(B)] - ab128873 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7314(B)
Overlay histogram showing Hela cells stained with ab128873 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128873, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CTCF antibody [mAbcam 37477] - ab37477 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 37477
All lanes : Anti-CTCF antibody [mAbcam 37477] (ab37477) at 5 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-CTCF antibody - C-terminal - ab136316 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CTCF antibody - C-terminal (ab136316) at 1/2000 dilutionLane 1 : Mouse stem cell cytoplasmic lysateLane 2 : Mouse stem cell nuclear lysateLysates/proteins at 20 µg per lane.Secondarystandard HRP-labelled anti-rabbit at 1/3000 dilutiondeveloped using the ECL technique

Anti-CTCF antibody - ChIP Grade - ab70303 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CTCF with ab70303 at 1/100 (2µg/ml). Detection: DAB.

Anti-CTCF Antibody (aa650-700) - LS-C287428 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse by Immunohistochemistry. Sample: FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- used at a dilution of 1:100 (2 Detection: DAB.

Anti-CTCF Antibody (C-Terminus) IHC-plus™ - LS-B10616 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Anti-CTCF antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10616 concentration 5 ug/ml.

Anti-CTCF Antibody (N-Terminus) IHC-plus™ - LS-B1518 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Anti-CTCF antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1518 concentration 5 ug/ml.

Anti-CTCF Antibody (aa150-200) IHC-plus™ - LS-B3184 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Anti-CTCF antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3184 concentration 5 ug/ml.

Anti-CTCF Antibody (aa650-700) - LS-C78024 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CTCF Antibody (clone 252C3a) - LS-C78023 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 252C3a

Anti-CTCF Antibody (C-Terminus) - LS-C78027 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CTCF Antibody (aa650-700) - LS-C78026 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
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