Anti-CTCF antibody - ChIP Grade
| Name | Anti-CTCF antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab70303 |
| Prices | $400.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ChIP IHC-P EMSA |
| Species Reactivities | Mouse, Human, Rat, Bovine, Pig, Chimpanzee, Monkey, Ape, Orangutan, Elephant |
| Antigen | Synthetic peptide corresponding to a region between residues 650 and 700 of human CTCF (Peptide available as ab115042 |
| Description | Rabbit Polyclonal |
| Gene | CTCF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CTCF with ab70303 at 1/100 (2µg/ml). Detection: DAB.
All lanes : Anti-CTCF antibody - ChIP Grade (ab70303) at 0.03 µg/mlLane 1 : MCF7 whole cell lysate at 50 µgLane 2 : MCF7 whole cell lysate at 15 µgLane 3 : MCF7 whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgLane 6 : HeLa whole cell lysate at 50 µg
Detection of Human CTCF by Western Blot of Immunprecipitate. Lane 1: MCF7 whole cell lysates immunoprecipitated using ab70302 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 2: MCF7 whole cell lysates immunoprecipitated using ab70303 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 3: MCF7 whole cell lysates immunoprecipitated using ab70304 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 4: control IgG was used for immunoprecipitation. All lanes: ab70303 at 0.03µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds.
Analysis of Human CTCF by Chromatin Immunoprecipitation (ChIP). Primary human fibroblasts from lung (5 x 107 cells) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 20µg ab70303. The resulting ChIP DNA was quantified using real-time PCR with primers against the IGF2-H19 ICR or a control region in the HoxA cluster.
Product References
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