CTCF (D31H2) XP ® Rabbit mAb

Name	CTCF (D31H2) XP ® Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	3418
Prices	$109.00, $277.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	D31H2
Applications	WB IP IHC-P ICC/IF ChIP
Species Reactivities	Human, Mouse, Rat, Monkey, Bovine, Horse
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human CTCF protein.
Description	Rabbit Monoclonal
Gene	CTCF
Supplier Page	Shop

Product images

Western blot analysis of extracts from various cell lines using CTCF (D31H2) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CTCF (D31H2) XP ® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CTCF (D31H2) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymphoma using CTCF (D31H2) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of HCT-116 cells using CTCF (D31H2) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Hela cells and 10 μl of CTCF (D31H2) XP ® Rabbit mAb, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5ng enriched ChIP DNA using NEBNext ® Ultra™ II DNA Library Prep Kit for Illumina ® , and sequenced on the Illumina NextSeq. The figure shows binding across H19, a known target gene of CTCF (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells, with either 10 μl of CTCF (D31H2) XP ® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human c-Myc promoter primers, SimpleChIP ® Human H19/Igf2 ICR Primers #5172, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.