CTCF (D1A7) XP ® Rabbit mAb
| Name | CTCF (D1A7) XP ® Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 3417 |
| Prices | $277.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D1A7 |
| Applications | WB IP ICC/IF ChIP |
| Species Reactivities | Human, Rat, Monkey, Bovine |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the human CTCF protein. |
| Description | Rabbit Monoclonal |
| Gene | CTCF |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using CTCF (D1A7) XP ® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using CTCF (D1A7) XP ® Rabbit mAb (green). Actin filaments are labeled with DY-554 phalloidin (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 μl of CTCF (D1A7) XP ® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human c-Myc promoter primers, SimpleChIP ® Human H19/Igf2 ICR Primers #5172, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.