CTCF Antibody
| Name | CTCF Antibody |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 2899 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | WB IP ICC/IF ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey |
| Antigen | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human CTCF protein |
| Description | Rabbit Polyclonal |
| Gene | CTCF |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using CTCF Antibody.
Confocal immunofluorescent analysis of HeLa cells using CTCF Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 20 μl of CTCF Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human c-Myc promoter primers, SimpleChIP ® Human H19/Igf2 ICR Primers #5172, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.