SPHK1

Gene Symbol SPHK1
Entrez Gene 8877
Alt Symbol SPHK
Species Human
Gene Type protein-coding
Description sphingosine kinase 1
Other Description SK 1|SPK 1
Swissprots Q9NYA1 Q9NY70 Q9HD92 Q96GK1 Q8N632 Q9NYL3
Accessions AAP35060 CAE90363 CAE90969 EAW89388 EAW89389 EAW89390 EAW89391 EAW89392 EAW89393 EAW89394 Q9NYA1 AF200328 AAG01980 AF238083 AAF73423 AF266756 AAF73470 AJ245504 CAB92131 AK022402 BAB14028 AK023393 BAB14558 AK095578 AK122781 BAG53725 AK225250 AK225258 AK292294 BAF84983 AK292614 BAF85303 BC004112 AAH04112 BC008040 AAH08040 BC009419 AAH09419 BC014439 AAH14439 BC030553 AAH30553 BP304783 DC384776 XM_005257766 XP_005257823 XM_011525439 XP_011523741 NM_001142601 NP_001136073 NM_001142602 NP_001136074 NM_021972 NP_068807 NM_182965 NP_892010
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. {ECO:0000269|PubMed:20577214, ECO:0000269|PubMed:23602659}.
Subcellular Location Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.
Tissue Specificity Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. {ECO:0000269|PubMed:10802064}.
Top Pathways VEGF signaling pathway, Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Tuberculosis, Sphingolipid signaling pathway

SPHK1 (D1H1L) Rabbit mAb - 12071 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1H1L
Western blot analysis of extracts from various cell lines using SPHK1 (D1H1L) Rabbit mAb.

SPHK1 Antibody - 3297 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using SPHK1 Antibody.

SphK1 (G-14) - sc-34528 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SphK1 (M-209) - sc-48825 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SphK1 (M-209): sc-48825. Western blot analysis of SphK1 expression in C2C12 (A), PC-12 (B) and RAW 264.7 (C) whole cell lysates and rat skeletal muscle (D) and mouse embryo (E) tissue extracts.

SphK1 (FQ-9) - sc-100441 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
SphK1 (FQ-9): sc-100441. Western blot analysis of SphK1 expression in A-431 whole cell lysate.

Anti-SPHK1 antibody - ab71700 from Abcam

Prices $401.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SPHK1 antibody (ab71700) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : HepG2 cell lysateLane 3 : HUVEC cell lysateLane 4 : Raji cell lysateLane 5 : C6 cell lysateLane 6 : C2C12 cell lysateLysates/proteins at 35 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

Anti-SPHK1 antibody - ab16491 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SPHK1 antibody (ab16491) at 1 µg/mlLane 1 : Tagged Recombinant SPHK1Lane 2 : Tagged Recombinant SPHK1 with Human SPHK1 peptide (ab16634) at 1 µg/mlLysates/proteins at 0.1 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

Anti-SPHK1 antibody - ab37980 from Abcam

Prices $379.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab37980 stained HepG2 cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37980, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SPHK1 antibody - ab77475 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-SPHK1 antibody (ab77475) at 2 µg/ml + human peripheral blood mononucleocyte lysate in RIPA buffer at 35 µg

Anti-SPHK1 antibody - ab56414 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SPHK1 antibody - ab172290 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-SPHK1 antibody (ab172290) at 1 µg/mlLane 1 : SPHK1 transfected 293T cell lysateLane 2 : Non-transfected cell lysateLysates/proteins at 15 µl per lane.

Anti-SPHK1 antibody - ab61148 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Ab61148 (10ug/ml) staining human SPHK1 in human adrenal tissue (pheochromocytes) by immunohistochemistry using formalin fixed, paraffin embedded tissue.

Anti-SPHK1 antibody - ab46719 from Abcam

Prices $384.00
Sizes 350 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SPHK1 antibody (ab46719) at 2 µg/mlLane 1 : Human platelet microsomes (20ug)Lane 2 : Murine kidney pellet (20ug)Lane 3 : Murine spleen supernatant( 40ug)Lane 4 : Raji cell lysate (40ug)

Anti-SPHK1 antibody [EPR4293(2)] - ab109522 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4293(2)
All lanes : Anti-SPHK1 antibody [EPR4293(2)] (ab109522) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : JAR cell lysateLane 3 : HUVEC cell lysateLane 4 : HT-1080 cell lysateLysates/proteins at 10 µg per lane.

Anti-SPHK1 antibody (FITC) - ab95400 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SPHK1 antibody - N-terminal - ab140944 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human skeletal muscle tissue labelling SPHK1 with ab140944.

Anti-SPHK / SPHK1 Antibody (aa59-89) - LS-C100770 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical of paraffin-embedded M. kidney section using SPHK1 Antibody(N-term P74). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Anti-SPHK / SPHK1 Antibody (aa1-30) - LS-C100768 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of anti-hSPHK1-M1 antibody in 293 cell line lysates transiently transfected with the SPHK1 gene (2 ug/lane). hSPHK1-M1(arrow) was detected using the purified antibody.

Anti-SPHK / SPHK1 Antibody - LS-C330808 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human stomach cancer using SPHK1 antibody at dilution of 1:200 (x400 lens).

Anti-SPHK / SPHK1 Antibody (aa286-315) - LS-C100769 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-SPHK / SPHK1 Antibody (aa313-343) - LS-C161448 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SPHK1 Antibody (C-term R414)IHC of formalin-fixed and paraffin-embedded skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the SPHK1 Antibody (C-term R414) for immunohistochemistry.

Anti-SPHK / SPHK1 Antibody (N-Terminus) IHC-plus™ - LS-A3104 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SPHK1 antibody LS-A3104 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-SPHK / SPHK1 Antibody (clone 1A6) - LS-C338427 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A6
IHC of paraffin-embedded Human endometrium tissue using anti-SPHK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-SPHK / SPHK1 Antibody (aa18-29) - LS-C3242 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SPHK / SPHK1 Antibody - LS-C26924 from LifeSpan Bioscience

Prices $460.00
Sizes 10 blot
Host Rabbit
Clonality Polyclonal
Western blot of rat kidney lysate (10 ug/lane), detection of endogenous Sphingosine kinase 1, long form using X1848P (1 ug/ml), HRP anti-rabbit was used at 1:75k, and developed with Pierce Super Signal West Femto.
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