Immunohistochemical of paraffin-embedded M. kidney section using SPHK1 Antibody(N-term P74). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical of paraffin-embedded H. kidney section using SPHK1 Antibody(N-term P74). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot of lysates from HepG2 cell line and human heart, skeletal muscle, kidney tissue lysate(from left to right), using SPHK1 Antibody (N-term P74). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.