Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
Western blot of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
The anti-SPHK1 antibody is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.
Western blot of anti-SPHK1 Antibody in 293 cell line lysates (35 ug/lane). SPHK1 (arrow) was detected using the purified antibody.