TNFSF11

Gene Symbol TNFSF11
Entrez Gene 8600
Alt Symbol CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf
Species Human
Gene Type protein-coding
Description tumor necrosis factor (ligand) superfamily, member 11
Other Description TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of nuclear factor kappa B ligand|receptor activator of nuclear factor kappa-B ligand|tumor necrosis factor ligand superfamily member 11
Swissprots Q9P2Q3 O14723 Q96Q17 O14788
Accessions CAJ09926 CAX24322 EAX08679 EAX08680 EAX08681 O14788 AA504211 AB037599 BAA90488 AB061227 BAB71768 AB064268 BAB79693 AB064269 BAB79694 AB064270 BAB79695 AF013171 AAC51762 AF019047 AAB86811 AF053712 AAC39731 BC040889 BC074823 AAH74823 BC074890 AAH74890 BC117286 AAI17287 BC117288 AAI17289 BQ007726 HQ258604 ADR83357 XM_011535280 XP_011533582 NM_003701 NP_003692 NM_033012 NP_143026
Function Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. {ECO:0000269|PubMed:22664871}.
Subcellular Location Isoform 2: Cytoplasm {ECO:0000250}.
Tissue Specificity Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
Top Pathways Cytokine-cytokine receptor interaction, Osteoclast differentiation, Rheumatoid arthritis, Prolactin signaling pathway

RANK Ligand (L300) Antibody - 4816 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with a construct expressing RANK Ligand, using RANK Ligand (L300) Antibody.

RANK Ligand (R2) Antibody - 3959 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected (+) with a construct expressing human RANK Ligand, using RANK Ligand (R2) Antibody

RANKL (G-1) - sc-377079 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RANKL (G-1): sc-377079. Western blot analysis of RANKL expression in non-transfected: sc-117750 (A) and human RANKL transfected: sc-110023 (B) CHO whole cell lysates.

RANKL (FL-317) - sc-9073 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RANKL (FL-317): sc-9073. Immunofluorescence staining of normal mouse heart frozen section showing membrane and cytoplasmic staining.

RANKL (N-19) - sc-7628 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RANKL (N-19): sc-7628. Western blot analysis of RANKL expression in BYDP whole cell lysate.

RANKL (C-20) - sc-7627 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RANKL (C-20): sc-7627. Western blot analysis of mouse recombinant RANKL.

RANKL (12A380) - sc-59982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

RANKL (4i167) - sc-71955 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

Anti-RANKL antibody - ab9957 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab9957 staining RANKL in human metastatic carcinoma of lymph nodes from breast cancer tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

Anti-RANKL antibody - ab65024 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab65024 at 1/500 - 1/1000 dilution staining RANKL in HepG2 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.

Anti-RANKL antibody - ab93719 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RANKL antibody (ab93719) at 1 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-RANKL antibody - ab194627 from Abcam

Prices $0.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-RANKL antibody - ab97863 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-RANKL antibody (ab97863) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-RANKL antibody - ab97864 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-RANKL antibody (ab97864) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

Anti-RANKL antibody - ab169966 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal colon tissue labeling RANKL with ab169966 at 1/100 dilution.

Anti-RANKL antibody - ab71282 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RANKL antibody - ab22113 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-RANKL antibody (ab22113) at 0.5 µg/ml + partial His-tagged recombinant protein Observed band size : 21 kDa (why is the actual band size different from the predicted?)

Anti-RANKL antibody [12A380] - ab12125 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 12A380
ICC/IF image of ab12125 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12125, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RANKL antibody [12A668] - ab45039 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 12A668
Human lymph node probed with ab45039 at 5 µg/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Anti-sRANKL antibody - ab62516 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-sRANKL antibody (ab62516) at 0.25 µg/mlLane 2 : Anti-sRANKL antibody (ab62516) at 0.5 µg/mlLane 1 : rat liver tissue lysateLane 2 : rat liver tissue lysateLysates/proteins at 15 µg per lane.

Anti-sRANKL antibody - ab189129 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-sRANKL antibody (ab189129) at 0.25 µg/mlLane 2 : Anti-sRANKL antibody (ab189129) at 0.5 µg/mlLane 1 : Rat liver tissue lysateLane 2 : Rat liver tissue lysate

Anti-sRANKL antibody (Biotin) - ab83145 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-sRANKL antibody (Biotin) - ab83436 from Abcam

Prices $370.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-sRANKL antibody - C-terminal - ab190642 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-sRANKL antibody - C-terminal (ab190642) at 1/500 dilution + 293 cell extract at 30 µg

Anti-TNFSF11 / RANKL / TRANCE Antibody (aa1-30) - LS-C160070 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TNFSF11 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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