Anti-RANKL antibody
| Name | Anti-RANKL antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9957 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | unknown |
| Applications | ICC/IF ICC/IF ELISA WB IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Highly pure (>98%) recombinant hsRANK-L (human soluble receptor activator of NF-Kappa B Ligand) |
| Description | Rabbit Polyclonal |
| Gene | TNFSF11 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab9957 staining RANKL in human metastatic carcinoma of lymph nodes from breast cancer tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
ICC/IF image of ab9957 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9957, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-RANKL antibody (ab9957) at 1 µg/ml + Spleen (Human) Tissue Lysate - adult normal tissue (ab29699) at 10 µgSecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Preferential recognition of isocitrate dehydrogenase by a rabbit monoclonal - Preferential recognition of isocitrate dehydrogenase by a rabbit monoclonal
Terasawa K, Rajapakshe AR, Podyma-Inoue KA, Mishima-Tsumagari C, Yanagishita M, Hara-Yokoyama M. J Immunol Methods. 2015 Mar 13. pii: S0022-1759(15)00070-8. doi:
Impact of the Oral Commensal Flora on Alveolar Bone Homeostasis. - Impact of the Oral Commensal Flora on Alveolar Bone Homeostasis.
Irie K, Novince CM, Darveau RP. J Dent Res. 2014 Jun 16;93(8):801-806.
Differential expression of the RANKL/RANK/OPG system is associated with bone - Differential expression of the RANKL/RANK/OPG system is associated with bone
Peng X, Guo W, Ren T, Lou Z, Lu X, Zhang S, Lu Q, Sun Y. PLoS One. 2013;8(3):e58361.
Adenosine A2A receptor activation prevents wear particle-induced osteolysis. - Adenosine A2A receptor activation prevents wear particle-induced osteolysis.
Mediero A, Frenkel SR, Wilder T, He W, Mazumder A, Cronstein BN. Sci Transl Med. 2012 May 23;4(135):135ra65.
The RANKL/RANK/OPG signaling pathway mediates medial arterial calcification in - The RANKL/RANK/OPG signaling pathway mediates medial arterial calcification in
Ndip A, Williams A, Jude EB, Serracino-Inglott F, Richardson S, Smyth JV, Boulton AJ, Alexander MY. Diabetes. 2011 Aug;60(8):2187-96.