RPS6KB1

Gene Symbol RPS6KB1
Entrez Gene 6198
Alt Symbol PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha
Species Human
Gene Type protein-coding
Description ribosomal protein S6 kinase, 70kDa, polypeptide 1
Other Description p70 S6 kinase, alpha|ribosomal protein S6 kinase I|ribosomal protein S6 kinase beta-1|serine/threonine kinase 14 alpha|serine/threonine-protein kinase 14A
Swissprots F6UYM1 E7ESB8 B4DTG1 B4DLT4 Q7Z721 B2R779 P23443
Accessions CAM82744 CDL93497 EAW94382 EAW94383 EAW94384 P23443 AA424792 AI806441 AK026730 AK091503 AK293247 BAG56781 AK297147 BAG59646 AK300202 BAG61973 AK312875 BAG35726 BC030763 BC036033 AAH36033 BC053365 AAH53365 BG614269 BP359158 BQ719717 CA444907 CB050838 M60724 AAA36410 XM_006722010 XP_006722073 XM_011525101 XP_011523403 XM_011525102 XP_011523404 XM_011525103 XP_011523405 XM_011525104 XP_011523406 NM_001272042 NP_001258971 NM_001272043 NP_001258972 NM_001272044 NP_001258973 NM_001272060 NP_001258989 NM_003161 NP_003152
Function Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein
Subcellular Location Isoform Alpha II: Cytoplasm.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:9804755}.
Top Pathways Fc gamma R-mediated phagocytosis, Proteoglycans in cancer, Choline metabolism in cancer, AMPK signaling pathway, PI3K-Akt signaling pathway

p70 S6 Kinase (49D7) Rabbit mAb (Biotinylated) - 5707 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 49D7
Western blot analysis of extracts from HeLa and PC-12 cells, using p70 S6 Kinase (49D7) Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999.

Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb - 9234 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 108D2
Western blot analysis of extracts from serum starved or serum treated (20%) 293, NIH/3T3, and PC12 cells, using Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb (upper), or p70 S6 Kinase (49D7) rabbit mAb #2708 (lower).

Phospho-p70 S6 Kinase (Thr389) Antibody - 9205 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of HeLa, COS, C6 and 3T3 cells, serum-starved overnight, then treated with insulin, lambda-phosphatase or 20% serum as indicated. Upper panel probed with Phospho-p70 S6 Kinase (Thr389) Antibody #9205; lower panel probed with p70 S6 Kinase Antibody #9202.

Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody - 9204 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated, serum-treated or EGF-treated (100 ng/ml), using Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody (upper) or p70 S6 Kinase Antibody #9202 (lower).

Phospho-p70 S6 Kinase (Ser371) Antibody - 9208 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysates from unsynchronized (U) and nocodazole (N) treated (50ng/ml for 48 hours) HT29 cells using Phospho-p70 S6 Kinase (Ser371) Antibody (B) and p70 S6 Kinase Antibody #9202 (D). Incubation of the nitrocellulose membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer abolishes the phospho-p70 S6 Kinase signal (A), but has no effect on the total p70 S6 kinase signal (C).

PhosphoPlus® p70 S6 Kinase (Thr389, Thr421/Ser424) Antibody Kit - 9430 from Cell Signaling Technology

Prices $489.00
Sizes 1 kit (10 western blots)
Clonality Polyclonal

Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb - 9206 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (20 western blots), 300 µl (60 western blots)
Host Mouse
Clonality Monoclonal
Clone 1A5
Western blot analysis of extracts from NIH/3T3 cells, serum-starved or serum-treated (20%) using Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb (upper) and p70 S6 Kinase Antibody #9202 (lower).

p70 S6 Kinase Antibody - 9202 from Cell Signaling Technology

Prices $246.00, $596.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH-3T3, PC12 and COS-7 cells using p70 S6 Kinase Antibody.

p70 S6 Kinase (49D7) Rabbit mAb - 2708 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 49D7
Western blot analysis of extracts from PC12, NIH/3T3, and SK-N-MC cells, using p70 S6 Kinase (49D7) Rabbit mAb.

Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb - 5260 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 1A5

p70 S6 kinase α (B-5) - sc-393967 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p70 S6 kinase α (B-5): sc-393967. Western blot analysis of p70 S6 kinase α expression in NIH/3T3 whole cell lysate.

p70 S6 kinase α (H-160) - sc-9027 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p70 S6 kinase α (H-160): sc-9027. Western blot analysis of p70 S6 kinase α expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.

p70 S6 kinase α (H-9) - sc-8418 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p70 S6 kinase α (H-9) PE: sc-8418 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

p70 S6 kinase α (C-18) - sc-230 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p70 S6 kinase α (C-18): sc-230. Western blot analysis of p70 S6 kinase α expression in HeLa (A), NIH/3T3 (B) and KNRK (C) whole cell lysates and rat liver tissue (D).

p-p70 S6 kinase α (E-5) - sc-377529 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A,D), EGF treated (B,E) and EGF and lambda protein phosphatase (sc-200312A) treated (C,F) MCF7 whole cell lysates. Antibodies tested include p-p70 S6 kinase α (E-5): sc-377529 (A,B,C) and p70 S6 kinase α (S-04): sc-100423 (D,E,F).

p-p70 S6 kinase α (Thr 389) - sc-11759 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-p70 S6 kinase α (Thr 389)-R: sc-11759-R. Western blot analysis of p70 S6 kinase α phosphorylation in non-transfected 293T: sc-117752 (A), mouse p70 S6 kinase α transfected 293T: sc-125770 (B) and NIH/3T3 (C) whole cell lysates.

p-p70 S6 kinase α (Thr 421) - sc-293113 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-p70 S6 kinase α (Thr 421): sc-293113. Western blot analysis of p70 S6 kinase α phosphorylation expression in untreated (A) and GM-CSF treated (B) Jurkat whole cell lysates.

p-p70 S6 kinase α (Ser 424) - sc-293112 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-p70 S6 kinase α (Ser 424): sc-293112. Western blot analysis of p70 S6 kinase α phosphorylation expression in untreated (A) and EGF treated (B) MCF7 whole cell lysates.

p-p70 S6 kinase α (Ser 411) - sc-101768 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-p70 S6 kinase α (Ser 411): sc-101768. Western blot analysis of phosphorylated p70 S6 kinase α expression in untreated (A) and Insulin-treated (B) 293 whole cell lysates.

Anti-P70 S6 Kinase alpha (phospho S371) antibody - ab195986 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-P70 S6 Kinase alpha (phospho S371) antibody (ab195986) at 1/500 dilutionLane 1 : whole lysate of COLO205 cells treated with serum with antigen-specific peptideLane 2 : whole lysate of COLO205 cells treated with serumLysates/proteins at 12.5 µg per lane.

Anti-P70 S6 Kinase alpha (phospho T229) antibody - ab59208 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilutionLane 1 : Jurkat cell extractLane 2 : Jurkat cell extract with immunizing phosphopeptide

Anti-P70 S6 Kinase alpha (phospho T229) antibody - ab195982 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab195982) at 1/500 dilutionLane 1 : Jurkat whole cell lysate with the antigen-specific peptideLane 2 : Jurkat whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-P70 S6 Kinase alpha (phospho T412) antibody - ab194521 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-P70 S6 Kinase alpha (phospho T412) antibody (ab194521) at 1/500 dilutionLane 1 : Insulin treated Jurkat cell extractLane 2 : Insulin treated Jurkat cell extract with antigen-specific peptide

Anti-P70 S6 Kinase alpha (phospho T421) antibody - ab196008 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-P70 S6 Kinase alpha (phospho T421) antibody (ab196008) at 1/500 dilutionLane 1 : EGF treated NIH 3T3 whole cell lysates with antigen-specific peptideLane 2 : EGF treated NIH 3T3 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-P70 S6 Kinase alpha antibody [EPR13429] - ab186753 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13429
All lanes : Anti-P70 S6 Kinase alpha antibody [EPR13429] (ab186753) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
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