HSF1

Gene Symbol HSF1
Entrez Gene 3297
Alt Symbol HSTF1
Species Human
Gene Type protein-coding
Description heat shock transcription factor 1
Other Description HSF 1|HSTF 1|heat shock factor protein 1
Swissprots A8MW26 A8K4L0 Q53XT4 Q00613
Accessions CAM91162 EAW82131 Q00613 AF299094 AAG23698 AK054762 BAG51421 AK125467 BAG54202 AK126580 AK222497 BAD96217 AK290975 BAF83664 AK300205 BAG61976 BC014638 AAH14638 BT007351 AAP36015 DA481620 DQ893859 ABM84785 M64673 AAA52695 XM_005272315 XP_005272372 XM_005272316 XP_005272373 XM_005272317 XP_005272374 XM_011517004 XP_011515306 XM_011517005 XP_011515307 XM_011517006 XP_011515308 XR_246618 NM_005526 NP_005517
Function DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. {ECO:0000269|PubMed:11447121, ECO:0000269|PubMed:11583998, ECO:0000269|PubMed:12659875, ECO:0000269|PubMed:12665592, ECO:0000269|PubMed:16278218, ECO:0000269|PubMed:8946918, ECO:0000269|PubMed:9121459, ECO:0000269|PubMed:9535852}.
Subcellular Location Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
Top Pathways Legionellosis

HSF1 (D3L8I) Rabbit mAb - 12972 from Cell Signaling Technology

Prices $250.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3L8I
Western blot analysis of extracts from various cell lines using HSF1 (D3L8I) Rabbit mAb.

HSF1 Antibody - 4356 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using HSF1 antibody.

HSF1 (A-7) - sc-393677 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSF1 (A-7): sc-393677. Western blot analysis of HSF1 expression in non-transfected 293T: sc-117752 (A), human HSF1 transfected 293T: sc-171930 (B) and HeLa (C) whole cell lysates.

HSF1 (C-9) - sc-393509 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSF1 (C-9): sc-393509. Western blot analysis of HSF1 expression in HeLa (A) and MDA-MB-231 (B) whole cell lysates.

HSF1 (C-19) - sc-8061 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HSF1 (C-19): sc-8061. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

HSF1 (10H8) - sc-13516 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
HSF1 (10H8): sc-13516. Western blot analysis of HSF1 expression in non-transfected: sc-117752 (A) and mouse HSF1 transfected: sc-120904 (B) 293T whole cell lysates.

HSF1 (C-5) - sc-17756 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSTF1 (C-5): sc-17756. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.

HSF1 (E-4) - sc-17757 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSF1 (E-4): sc-17757. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.

HSF1 (H-311) - sc-9144 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HSF1 (H-311): sc-9144. Western blot analysis of HSF1 expression in PC-12 (A) and F9 (B) whole cell lysates.

p-HSF1 (Ser 303) - sc-101698 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated HSF1 expression in MCF7 whole cell lysates (A,B). Blots were probed with p-HSF1 (Ser 303): sc-101698 preincubated with its cognate phosphorylated peptide (A) and p-HSF1 (Ser 303): sc-101698 (B).

p-HSF1 (Ser 307) - sc-135640 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated HSF1 expression in TNF-α-treated HUVEC cell extracts. Blots were probed with p-HSF1 (Ser 307): sc-135640 preincubated with cognate phosphorylated peptide (A) and p-HSF1 (Ser 307): sc-135640 (B).

Anti-HSF1 (phospho S121) antibody - ab79269 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-HSF1 (phospho S121) antibody (ab79269) at 1/500 dilutionLane 1 : Jurkat cell extract, treated with TNF (2500U/ML, 30mins)Lane 2 : Jurkat cell extract, treated with TNF (2500U/ML, 30mins) with immunising phosphopeptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - ab81281 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1711Y
All lanes : Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281) at 1/50000 dilutionLane 1 : HeLa cell lysate untreatedLane 2 : HeLa cell lysate AP treatedLysates/proteins at 10 µg per lane.SecondaryHRp labelled goat anti rabbit at 1/2000 dilution

Anti-HSF1 (phospho S303) antibody - ab47369 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lanes 1 - 2 : HSF1 antibody at 1/500 dilutionLanes 3 - 4 : Anti-HSF1 (phospho S303) antibody (ab47369) at 1/500 dilutionLane 1 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). No peptide. Lane 2 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). Synthetic phosphopeptide present. Lane 3 : Extracts from MCF cells not treated with TNF alpha. No peptide. Lane 4 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). No peptide.

Anti-HSF1 (phospho S303) antibody - ab105084 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HSF1 (phospho S303) antibody (ab105084) at 1 µg/mlLane 1 : 30min TNFalpha stimulated MEF Whole Cell LysateLane 2 : 30min TNFalpha stimulated MEF Whole Cell Lysate with Immunizing peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HSF1 (phospho S307) antibody - ab75905 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab75905 at 1/100 dilution staining HSF1 in methanol-fixed HeLa cells by Immunofluorescence.

Anti-HSF1 (phospho S320) antibody [EPR1712] - ab76183 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1712
Immunohistochemical analysis of paraffin-embedded human muscles using ab76183 at 1/100 dilution.

Anti-HSF1 (phospho S326) antibody - ab115702 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HSF1 (phospho S326) antibody (ab115702) at 1/1000 dilutionLane 1 : MW markerLane 2 : HeLa cell lysateLane 3 : HeLa cell lysate Heat-Shocked (No recovery time)

Anti-HSF1 (phospho S326) antibody [EP1713Y] - ab76076 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1713Y
Overlay histogram showing HeLa cells stained with ab76076 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76076, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HSF1 (phospho T142) antibody - ab111425 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111425, at 1/50 dilution, staining HSF1 in paraffin-embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-HSF1 antibody - ab131081 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HSF1 antibody (ab131081) at 0.5 µg/mlLane 1 : Hela Cell LysateLane 2 : 293T Cell LysateLane 3 : CEM Cell LysateLane 4 : Raji Cell Lysate

Anti-HSF1 antibody - ab82586 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-HSF1 antibody (ab82586) at 1/1000 dilution + Human HSF1 full length protein (ab78795) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds

Anti-HSF1 antibody - ab47484 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Staining of HSF1 on paraffin-embedded human breast carcinoma tissue using ab47484 diluted 1/50. Right panel: the anibody was incubated with the corresponding blocking peptide.

Anti-HSF1 antibody - ab105086 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HSF1 antibody (ab105086) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HSF1 antibody - ab49995 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-HSF1 antibody (ab49995) at 1 µg/mlLane 2 : Anti-HSF1 antibody (ab49995) at 0.5 µg/mlLane 3 : Anti-HSF1 antibody (ab49995) at 0.25 µg/mlLane 1 : HeLa cell LysateLane 2 : HeLa cell LysateLane 3 : HeLa cell LysateSecondaryGoat Anti-Rabbit IgG-Peroxidase conjugate
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