Anti-HSF1 (phospho S303) antibody
| Name | Anti-HSF1 (phospho S303) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab47369 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthesized phosphopeptide derived from HSF1 around the phosphorylation site of serine 303 (Human) |
| Description | Rabbit Polyclonal |
| Gene | HSF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lanes 1 - 2 : HSF1 antibody at 1/500 dilutionLanes 3 - 4 : Anti-HSF1 (phospho S303) antibody (ab47369) at 1/500 dilutionLane 1 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). No peptide. Lane 2 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). Synthetic phosphopeptide present. Lane 3 : Extracts from MCF cells not treated with TNF alpha. No peptide. Lane 4 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). No peptide.
ab47369 (1/50) staining HSF1 in paraffin-embedded human breast carcinoma tissue. Right-hand panel corresponds to a negative control for which ab47369 was pre-incubated with the immunising (blocking) peptide.
ICC/IF image of ab47369 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47369, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
The master regulator of the cellular stress response (HSF1) is critical for - The master regulator of the cellular stress response (HSF1) is critical for
Filone CM, Caballero IS, Dower K, Mendillo ML, Cowley GS, Santagata S, Rozelle DK, Yen J, Rubins KH, Hacohen N, Root DE, Hensley LE, Connor J. PLoS Pathog. 2014 Feb 6;10(2):e1003904.
HSF1 regulation of beta-catenin in mammary cancer cells through control of - HSF1 regulation of beta-catenin in mammary cancer cells through control of
Chou SD, Murshid A, Eguchi T, Gong J, Calderwood SK. Oncogene. 2015 Apr 23;34(17):2178-88.
Deciphering human heat shock transcription factor 1 regulation via - Deciphering human heat shock transcription factor 1 regulation via
Batista-Nascimento L, Neef DW, Liu PC, Rodrigues-Pousada C, Thiele DJ. PLoS One. 2011 Jan 6;6(1):e15976.