H2AFX

Gene Symbol H2AFX
Entrez Gene 3014
Alt Symbol H2A.X, H2A/X, H2AX
Species Human
Gene Type protein-coding
Description H2A histone family, member X
Other Description H2AX histone|histone H2A.x|histone H2AX
Swissprots Q6IAS5 P16104 Q4ZGJ7
Accessions AAY22178 EAW67451 P16104 BC004915 AAH04915 BC011694 AAH11694 BC013416 AAH13416 CR457079 CAG33360 X14850 CAA32968 NM_002105 NP_002096
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. {ECO:0000269|PubMed:10959836, ECO:0000269|PubMed:12419185, ECO:0000269|PubMed:12607005, ECO:0000269|PubMed:15201865}.
Subcellular Location Nucleus. Chromosome.
Top Pathways Systemic lupus erythematosus, Alcoholism

Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 488 Conjugate) - 9719 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 20E3
Flow cytometric analysis of Jurkat cells, untreated (green) or etoposide-treated (blue), using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 488 Conjugate).

Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 9720 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 20E3
Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or etoposide-treated (right), double-labeled with Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue) and beta-Tubulin Antibody #2146 (red).

Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 8228 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 20E3
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate) #3623 (green).

Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Biotinylated) - 4309 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 20E3
Western blot analysis of extracts from HT-29 cells, untreated or UV-treated, using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999.

Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (PE Conjugate) - 5763 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 20E3
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (PE Conjugate).

Phospho-Histone H2A.X (Ser139) Antibody - 2577 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated or UV-treated, using Phospho-Histone H2A.X (Ser139) Antibody (upper) or Histone H2A Antibody #2572 (lower).

Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb - 9718 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 20E3
Western blot analysis of extracts from untreated or UV-treated 293 cells, using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (upper) or Histone H2A.X Antibody #2595 (lower).

Histone H2A.X (D17A3) XP ® Rabbit mAb - 7631 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D17A3
Western blot analysis of extracts from various cell lines using Histone H2A.X (D17A3) XP ® Rabbit mAb.

Histone H2A.X Antibody - 2595 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated, UV-treated (50 mJ for 2 hours), or sodium chromate-treated (6 uM for 2 or 24 hours), using Phospho-Histone H2A.X (Ser139) Antibody #2577 (upper) or Histone H2A.X Antibody (lower).

Phospho-Histone H2A.X (Ser139/Tyr142) Antibody - 5438 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated or UV-treated, using Phospho-Histone H2A.X (Ser139/Tyr142) Antibody (upper) or Histone H2A.X Antibody #2595 (lower).

Histone H2A.X (C-20) - sc-54606 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Histone H2A.X (C-20): sc-54606. Western blot analysis of Histone H2A.X expression in non-transfected: sc-117752 (A) and mouse Histone H2A.X transfected: sc-120804 (B) 293T whole cell lysates.

p-Histone H2A.X (Ser 139) - sc-101696 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Histone H2A.X (Ser 139): sc-101696. Western blot analysis of phosphorylated Histone H2A.X expression in untreated (A) and UV-treated (B) 293 whole cell lysates.

Anti-gamma H2A.X (phospho S139) antibody - ab11174 from Abcam

Prices $403.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right) labelling H2A.X (phospho S139 with ab11174 at 1/5000 (0.2µg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.

Anti-gamma H2A.X (phospho S139) antibody [9F3] - ab26350 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 9F3
All lanes : Anti-gamma H2A.X (phospho S139) antibody [9F3] (ab26350) at 1/1000 dilutionLane 1 : Untreated Zebrafish embryos.Lane 2 : Zebrafish embryos treated with 25 Gy of X-Ray.Lane 3 : Zebrafish embryos treated with 50 Gy of X-RaySecondaryGoat anti-mouse HRP at 1/10000 dilutionThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-gamma H2A.X (phospho S139) antibody - ChIP Grade - ab2893 from Abcam

Prices $403.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab2893 staining γH2A.X in MALME-3M cells treated with terfenadine (ab120270), by ICC/IF. Increase of γH2A.X nuclear expression correlates with increased concentration of terfenadine, as described in literature.The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab120270 (terfenadine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-gamma H2A.X (phospho S140) antibody [3F2] - ab22551 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F2
ab22551 labelling gamma H2A.X (phospho S140) in HeLa cells by immunocytochemistry/immunofluorescence.

Anti-Histone H2A.X (acetyl K5) antibody - ab129217 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab129217 stained HeLa cells. The cells were exposed to 2000µJ/cm2 of UV, incubated at 37˚C for a further 24 hours. The cells were then 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab129217, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Histone H2A.X (phospho S139) antibody - ab72022 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2A.X (phospho S139) antibody (ab72022) at 1/5000 dilutionLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate treated with camptothecin

Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] (Alexa Fluor® 647) - ab195189 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP854(2)Y
ab195189 staining Histone H2A.X in Jurkat cells. The cells were incubated with 25uM ETP for 5 hours (Treated) or solvent-only for control purposes (Non-treated). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195189 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/200 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade - ab81299 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP854(2)Y
Immunohistochemical staining of paraffin embedded human brain with purified ab81299 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade (Alexa Fluor® 488) - ab195188 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP854(2)Y
ab195188 staining Histone H2A.X in Jurkat cells. The cells were incubated with 25uM ETP for 5 hours (Treated) or solvent-only for control purposes (Non-treated). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195188 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade (HRP) - ab195190 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP854(2)Y
IHC image of Histone H2A.X staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195190, 1/71.4285714285714 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Histone H2A.X antibody - ab188819 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2A.X antibody (ab188819) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : 293 cell lysate

Anti-Histone H2A.X antibody - ab140498 from Abcam

Prices $384.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling Histone H2A.X with ab140498 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Histone H2A.X antibody - ab131492 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of methanol-fixed HeLa cells labelling Histone H2A.X using ab131492, at 1/100 dilution.
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