KRT14

Gene Symbol KRT14
Entrez Gene 3861
Alt Symbol CK14, EBS3, EBS4, K14, NFJ
Species Human
Gene Type protein-coding
Description keratin 14, type I
Other Description CK-14|cytokeratin 14|cytokeratin-14|keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)|keratin, type I cytoskeletal 14|keratin-14
Swissprots Q14715 Q9BUE3 P02533 Q9UBN2 Q9UBN3 Q9UCY4 Q53XY3
Accessions AAB59562 AAF04034 AAF04035 AAF04036 AAF04037 AAF04038 AAF04039 EAW60745 EAW60746 EAW60747 EAW60748 P02533 AK309280 BC002690 AAH02690 BC019097 AAH19097 BC042437 AAH42437 BC094830 AAH94830 BT007186 AAP35850 DB018715 DQ890733 ABM81659 DQ892997 ABM83923 DQ893905 ABM84831 M28646 AAA96689 NM_000526 NP_000517
Function The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. {ECO:0000269|PubMed:11724817}.
Subcellular Location Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern.
Tissue Specificity Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. {ECO:0000269|PubMed:9457912}.

Cytokeratin 14 (C-14) - sc-17104 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cytokeratin 14 (C-14)): sc-17104 Western blot analysis of Cytokeratin 14 expression in 804G whole cell lysate.

Cytokeratin 14 (RCK107) - sc-23878 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cytokeratin 14 (RCK107): sc-23878. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.

Cytokeratin 14 (LL001) - sc-53253 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cytokeratin 14 (LL001): sc-53253. Western blot analysis of Cytokeratin 14 expression in 804G whole cell lysate.

Cytokeratin 14 (SPK-14) - sc-58725 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Cytokeratin 14 (LL002) - sc-58724 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

Anti-Cytokeratin 14 [EP1612Y] antibody (Alexa Fluor® 488) - ab192055 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1612Y
Overlay histogram showing A431 cells stained with ab192055 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192055, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in A431 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

Anti-Cytokeratin 14 [EP1612Y] antibody (Alexa Fluor® 647) - ab192056 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1612Y
ab192056 staining Cytokeratin 14 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192056 at a working dilution of 1 in 100 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 488 Goat anti-Mouse secondary (ab150117) at 2μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 4% formaldehyde (10min) fixed HeLa cells under the same testing conditions.

Anti-Cytokeratin 14 antibody - ab198167 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Cytokeratin 14 with ab198167 at 1/25.

Anti-Cytokeratin 14 antibody - ab15461 from Abcam

Prices $379.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
Human Skin stained with anti-Cytokeratin 14 antibody, ab15461 by Immunohistochemistry (FFPE-sections).

Anti-Cytokeratin 14 antibody - ab48576 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab48576 staining Cytokeratin 14 in Human squamous cell carcinoma by immunohistochemistry using formalin fixed paraffin embedded tissue.

Anti-Cytokeratin 14 antibody - ab48937 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Cytokeratin 14 antibody (ab48937) at 1.25 µg/ml + Thymus lysate at 10 µg

Anti-Cytokeratin 14 antibody [CKB1] - ab49747 from Abcam

Prices $390.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone CKB1
ab49747 at a 1/100 dilution staining Cytokeratin 14 in human epidermal keratinocytes by Immunocytochemistry/ Immunofluorescence, incubated for 12 hours at +4°C. PFA fixed. Permeabilized with Triton. blocked with 10% milk for 1 hour at 16°C. Secondary used at a 1/200 dilution Goat anti-mouse IgM conjugated to Alexa Fluor 488.See Abreview

Anti-Cytokeratin 14 antibody [EP1612Y] - ab51054 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1612Y
ICC/IF image of ab51504 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51504, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cytokeratin 14 antibody [EP1612Y] (HRP) - ab192081 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1612Y
Anti-Cytokeratin 14 antibody [EP1612Y] (HRP) (ab192081) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Cytokeratin 14 antibody [EPR1612] - ab108417 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1612
Anti-Cytokeratin 14 antibody [EPR1612] (ab108417) at 1/10000 dilution + Human skin lysate at 10 µg

Anti-Cytokeratin 14 antibody [EPR17336] - ab197893 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17336
All lanes : Anti-Cytokeratin 14 antibody [EPR17336] (ab197893) at 1/50000 dilutionLane 1 : Mouse skin lysate at 20 µgLane 2 : Rat skin lysate at 20 µgLane 3 : Rat brain lysate at 10 µgLane 4 : Rat heart lysate at 10 µgLane 5 : Rat kidney lysate at 10 µgLane 6 : Rat spleen lysate at 10 µgLane 7 : C6(Rat glial tumor cells) whole cell lysate at 10 µgLane 8 : Raw264.7(Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate at 10 µgLane 9 : PC-12(Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µgLane 10 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Cytokeratin 14 antibody [EPR17350] - ab181595 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17350
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Basal cells of epidermis show strong staining while no staining on the stratum corneum. Counter stained with Hematoxylin.Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP Polymer for Rabbit/Mouse IgG.

Anti-Cytokeratin 14 antibody [LL002] - ab7800 from Abcam

Prices $401.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone LL002
ab7800 was used to stain mouse prostate.

Anti-Cytokeratin 14 antibody [LL002] - ab49806 from Abcam

Prices $385.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone LL002
ICC/IF image of ab49806 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49806, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cytokeratin 14 antibody [LL002] - ab80524 from Abcam

Prices $376.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone LL002
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human squamous cell lung carcinoma tissue using 2µg/ml ab80519 with a peroxidase-conjugated secondary antibody and a DAB chromogen. Note cytoplasmic staining of tumor cells.

Anti-Cytokeratin 14 antibody [LL002] (Biotin) - ab79029 from Abcam

Prices $376.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone LL002
ab79029 at 2µg/ml staining Cytokeratin 14 in human squamous cell carcinoma of lung by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells. The purified form of this antibody was used for staining.

Anti-Cytokeratin 14 antibody [LL002], prediluted - ab49911 from Abcam

Prices $372.00
Sizes 5 ml
Host Mouse
Clonality Monoclonal
Clone LL002

Anti-Cytokeratin 14 antibody, prediluted - ab15462 from Abcam

Prices $378.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Human skin stained with anti-cytokeratin 14 antibody, ab15462 by Immunohistochemistry (FFPE-sections).

Anti-Cytokeratin 14 antibody, prediluted - ab82756 from Abcam

Prices $370.00
Sizes 6 ml
Host Rabbit
Clonality Polyclonal

Anti-Cytokeratin 14 antibody [RCK107] - ab9220 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone RCK107
ICC/IF image of ab9220 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9220, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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