Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Squamous carcinoma cells show strong staining. Counter stained with Hematoxylin.Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP Polymer for Rabbit/Mouse IgG.

Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Basal cells of epidermis show strong staining while no staining on the stratum corneum. Counter stained with Hematoxylin.Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP Polymer for Rabbit/Mouse IgG.

Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Basal cells of epidermis show strong staining while no staining on the stratum corneum. Counter stained with Hematoxylin.Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP Polymer for Rabbit/Mouse IgG.

Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized PC-12 (Rat adrenal gland pheochromocytoma cells) labeling Cytokeratin 14 with ab181595 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on PC-12 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls:--ve control 1: ab181595 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

All lanes : Anti-Cytokeratin 14 antibody [EPR17350] (ab181595) at 1/20000 dilutionLane 1 : A431 (Human epidermoid carcinoma) whole cell lysateLane 2 : HaCaT (Human keratinocyte cells) whole cell lysateLane 3 : Mouse skin lysateLane 4 : Rat skin lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-Cytokeratin 14 antibody [EPR17350] (ab181595) at 1/20000 dilution + Human skin lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution