ab7800 staining Cytokeratin 14 in Human normal skin tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% Serum for 30 minutes at 21°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). The sample was incubated with primary antibody (1/100 in PBS + 0.5% Tween-20 + 0.5% BSA)) at 21°C for 30 minutes. An undiluted HRP-conjugated goat polyclonal to mouse IgG was used as secondary antibody.See Abreview

Anti-Cytokeratin 14 antibody [LL002] (ab7800) at 2 µg/ml + Human HaCaT whole cell lysate at 30 µgSecondaryGoat Anti-mouse IgG Polyclonal at 1/20000 dilutiondeveloped using the ECL techniqueObserved band size : 55 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview

Overlay histogram showing A431 cells stained with ab7800 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7800, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

All lanes : Anti-Cytokeratin 14 antibody [LL002] (ab7800) at 2 µg/mlLane 1 : Human Cytokeratin 14 full length protein (ab73637) at 0.1 µgLane 2 : Human Cytokeratin 14 full length protein (ab73637) at 0.01 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds

Immunohistochemical analysis of mouse mammary duct tissue, staining Cytokeratin 14 (red) with ab7800. Antigen retrieval was carried out on paraffin-embedded sections by boiling in citrate buffer (pH 6) for 18 minutes in a microwave. Sections were then blocked for 1.5 hours in blocking reagents, before incubating with primary antibody (0.26 µg/ml) overnight at 4°C. An AlexaFluor®555-conjugated goat anti-mouse IgG was used as the secondary antibody

Immunofluorescence analysis of mouse mammary epithelial cells, staining Cytokeratin 14 (red) with ab7800.