All lanes : Anti-Cytokeratin 14 antibody [EPR17336] (ab197893) at 1/50000 dilutionLane 1 : Mouse skin lysate at 20 µgLane 2 : Rat skin lysate at 20 µgLane 3 : Rat brain lysate at 10 µgLane 4 : Rat heart lysate at 10 µgLane 5 : Rat kidney lysate at 10 µgLane 6 : Rat spleen lysate at 10 µgLane 7 : C6(Rat glial tumor cells) whole cell lysate at 10 µgLane 8 : Raw264.7(Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate at 10 µgLane 9 : PC-12(Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µgLane 10 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-Cytokeratin 14 antibody [EPR17336] (ab197893) at 1/50000 dilution + human fetal skin lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Humansquamous cell carcinoma of cervix tissue labeling Cytokeratin 14 using ab197893 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab197893 and secondary antibody only.Note: Cytoplasm staining on human squamous cell carcinoma of cervix tissue was observed.
Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling Cytokeratin 14 using ab197893 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab197893 and secondary antibody only.Note: Cytoplasm staining on mouse skin tissue was observed.