FC Flow cytometry



K562 cells (target cells) were labelled with CFSE and co-cultured with NK cells (effector cells) at the ratios indicated above each panel for four hours at 37°C. At the end of co-incubation, dead cells were labeled with 7-AAD. The percentage of K562 cell death was analyzed by flow cytometry. When cultured alone, there is little cell death (2%) on the K562 cell population. Addition of NK cells at a ratio as low as 6.25:1 caused a significant increase in K562 cell death (8.2%). At a ratio of NK cells to target cells of 25:1, there is 19.7% death of K562 cells.

Aggresome Detection Kit - ab139486 from Abcam

Category Cellular Assay
Prices $330.00
Sizes 100 tests
The increase in the fluorescence intensity of the Aggresome Detection Reagent with the addition of MG-132 in Jurkat cells. Jurkat cells were mock induced with 0.08% DMSO (blue line) or induced with 8 μM MG-132 (black line) in a 37ºC, 5% CO2 incubator for 24 hours.

Annexin V Unlabeled Apoptosis Reagent - ab14193 from Abcam

Category Cellular Assay
Prices $335.00
Sizes 100 µg

Annexin V-Biotin Apoptosis Detection Kit - ab14190 from Abcam

Category Cellular Assay
Prices $489.00
Sizes 100 tests

Annexin V-Cy3 Apoptosis Detection Kit - ab14142 from Abcam

Category Cellular Assay
Prices $505.00
Sizes 100 tests

Annexin V-Cy3 Apoptosis Detection Kit with SYTOX - ab14144 from Abcam

Category Cellular Assay
Prices $510.00
Sizes 100 tests

Annexin V-Cy5 Apoptosis Detection Kit - ab14150 from Abcam

Category Cellular Assay
Prices $505.00
Sizes 100 tests

Annexin V-EGFP Apoptosis Detection Kit - ab14153 from Abcam

Category Cellular Assay
Prices $505.00
Sizes 100 tests

Annexin V-FITC Apoptosis Detection Kit - ab14085 from Abcam

Category Cellular Assay
Prices $505.00
Sizes 100 tests
Annexin V-FITC/ PI staining of AG06173 primary fibroblasts.105 cells were used for analysis. Resuspended cells were incubated with Annexin V-FITC for 15 min in the dark. Propidium iodide was used as a counterstain to discriminate necrotic/ dead cells from apoptotic cells. Left: negative control - AG6173 untreated cells. Right: positive control - AG6173 cells irradiated at 10 Gy.Image courtesy of S. Khoronenkova PhD, Gray Institute for Radiation Oncology and Biology, University of Oxford, Oxford, UK.

Annexin V-FITC Apoptosis Detection Kit with SYTOX - ab14086 from Abcam

Category Cellular Assay
Prices $510.00
Sizes 100 tests

Annexin V-PE Apoptosis Detection Kit - ab14155 from Abcam

Category Cellular Assay
Prices $515.00
Sizes 100 tests
Annexin V detected in 10e4 control Jurkat cells (black) or in cells treated with 50 μM H2O2 (Sigma) for 6 hours (red).

Annexin V-PE Apoptosis Detection Kit With SYTOX - ab39674 from Abcam

Category Cellular Assay
Prices $525.00
Sizes 100 tests

Annexin V-PE-Cy5 Apoptosis Detection Kit - ab14159 from Abcam

Category Cellular Assay
Prices $577.00
Sizes 100 tests

Apoptosis/ Necrosis Detection Kit (blue, green, red) - ab176749 from Abcam

Category Cellular Assay
Prices $309.00
Sizes 100 tests
Fluorescent analysis showing cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (green, Apopxin Green Indicator), and necrotic (red, indicated by 7-AAD staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescent microscope through the Violet, FITC and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown.

Apoptosis/ Necrosis Detection Kit (blue, red, green) - ab176750 from Abcam

Category Cellular Assay
Prices $361.00
Sizes 100 tests
The fluorescence image shows cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (red, stained by Apopxin Deep Red Indicator), and necrotic (green, indicated by Nuclear Green DCS1staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescence microscope through the Violet, Cy5 and FITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Triple staining of staurosporine-induced cells.

Autophagy Detection Kit - ab139484 from Abcam

Category Cellular Assay
Prices $467.00
Sizes 1 kit
Fluorescent microscopy analysis showing nucleus (blue nuclear stain; DAPI filter) and autophagic vesicules (green, FITC filter) in control HepG2 cells or cells treated with 1 uM Rapamycin (ab120224) for 24 hours.

Caspase 12 (active) FITC Staining Kit - ab65610 from Abcam

Category Cellular Assay
Prices $227.00, $530.00
Sizes 25 tests, 100 tests

Caspase 2 (active) FITC Staining Kit - ab65612 from Abcam

Category Cellular Assay
Prices $530.00
Sizes 100 tests

Caspase 3 (active) FITC Staining Kit - ab65613 from Abcam

Category Cellular Assay
Prices $530.00
Sizes 100 tests
Caspase 3 in Jurkat cells following 24 hour exposure to 2 uM Camptothecin (CPT; ab120115) with or without 20 nM caspase inhibitor Z-VAD(OMe)-FMK (zVAD; ab120487). Background signal subtracted, duplicates; +/- SD.

Caspase 3 (active) Red Staining Kit - ab65617 from Abcam

Category Cellular Assay
Prices $530.00
Sizes 100 tests
Caspase 3 activity in Jurkat cells (3 x10e5 cells) following 24 hour exposure to 2 uM Camptothecin (ab120115) with or without 50 μM caspase inhibitor Z-VAD(OMe)-FMK (ab120487). Background signal subtracted, duplicates; +/- SD.

Caspase 8 (active) FITC Staining Kit - ab65614 from Abcam

Category Cellular Assay
Prices $530.00
Sizes 100 tests
Active caspase 8 in control Jurkat cells (10e6/mL) or cells treated for five hours with 10 ug/mL Cyclohexamide (CHX) (ab120093) or four hours with 25 ug/mL Mitomycin C (MitoC) (ab120797). Background signal subtracted, duplicates; +/- SD.

Caspase 8 (active) Red Staining Kit - ab65618 from Abcam

Category Cellular Assay
Prices $530.00
Sizes 100 tests

Caspase 9 (active) FITC Staining Kit - ab65615 from Abcam

Category Cellular Assay
Prices $530.00
Sizes 100 tests

Caspase 9 (active) Red Staining Kit - ab65619 from Abcam

Category Cellular Assay
Prices $530.00
Sizes 100 tests

Caspase Cascade Screening Kit - ab102499 from Abcam

Category Cellular Assay
Prices $500.00
Sizes 100 tests
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