USP10

Gene Symbol USP10
Entrez Gene 9100
Alt Symbol UBPO
Species Human
Gene Type protein-coding
Description ubiquitin specific peptidase 10
Other Description deubiquitinating enzyme 10|ubiquitin carboxyl-terminal hydrolase 10|ubiquitin specific protease 10|ubiquitin thioesterase 10|ubiquitin thiolesterase 10|ubiquitin-specific-processing protease 10
Swissprots Q9BWG7 Q14694 B2RDJ8 B4DS84 Q9NSL7
Accessions CBX53857 EAW95470 EAW95471 Q14694 AK055375 AK293304 BAG56825 AK299618 BAG61546 AK315570 BAG37945 AL162049 CAB82392 BC000263 AAH00263 BC064516 AAH64516 BX537402 CAD97644 CR749515 CAH18330 D80012 BAA11507 DB077919 DQ892553 ABM83479 DQ895764 ABM86690 GD151792 XM_006721330 XP_006721393 XM_006721331 XP_006721394 XM_006721332 XP_006721395 XM_011523440 XP_011521742 XM_011523441 XP_011521743 XM_011523442 XP_011521744 XM_011523443 XP_011521745 NM_001272075 NP_001259004 NM_005153 NP_005144 NR_073577 NR_073578
Function Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34- containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. {ECO:0000269|PubMed:11439350, ECO:00
Subcellular Location Cytoplasm. Nucleus. Early endosome. Note=Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:11439350}.

USP10 Antibody - 5553 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells, either mock transfected or transfected with a human USP10 construct, using USP10 Antibody.

USP10 (D7A5) Rabbit mAb - 8501 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7A5
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP10 (hUSP10-Myc/DDK, +), using USP10 (D7A5) Rabbit mAb.

USP10 (C-7) - sc-365828 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
USP10 (C-7): sc-365828. Western blot analysis of USP10 expression in non-transfected: sc-117752 (A) and mouse USP10 transfected: sc-124489 (B) 293T whole cell lysates.

USP10 (H-112) - sc-367736 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

USP10 (P-18) - sc-79298 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
USP10 (P-18): sc-79298. Western blot analysis of USP10 expression in non-transfected: sc-117752 (A) and mouse USP10 transfected: sc-124489 (B) 293T whole cell lysates.

USP10 (C-20) - sc-79296 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

USP10 (T-20) - sc-79299 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-USP10 antibody - ab70895 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling USP10 with ab70895 at 1/1000 (0.2µg/ml). Detection: DAB.

Anti-USP10 antibody - ab72486 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-USP10 antibody (ab72486) at 1/1000 dilutionLane 1 : HEK293Lane 2 : HEK293/USP10

Anti-USP10 antibody - ab117606 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab117606, at 5 µg/ml, staining USP10 in Formalin-fixed, Paraffin-embedded Human Brain (cortex) tissue by Immunohistochemistry.

Anti-USP10 antibody - ab56433 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56433 USP10 antibody at 1ug/ml.

Anti-USP10 antibody [EPR4261] - ab109219 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4261
ICC/IF image of ab109219 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109219 at 1/50 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-USP10 Antibody (aa50-100) - LS-C286435 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human USP10 by Immunohistochemistry. Sample: FFPE section of human lung adenocarcinoma. Antibody: Affinity purified rabbit anti-USP10 used at a dilution of 1:100.

Anti-USP10 Antibody (aa500-529) - LS-C157333 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
USP10 Antibody western blot of SK-BR-3 cell line lysates (35 ug/lane). The USP10 antibody detected the USP10 protein (arrow).

Anti-USP10 Antibody (aa300-3500) - LS-C287738 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human USP10 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP10 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-USP10 Antibody (Internal, clone EPR4261) - LS-C137948 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4261
IHC of paraffin-embedded human kidney tissue using anti-USP10 antibody.

Anti-USP10 Antibody (aa50-100) - LS-C287739 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human USP10 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP10 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

Anti-USP10 Antibody (clone 1H9) - LS-C173574 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H9
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody.

Anti-USP10 Antibody (clone 1A10) - LS-C173656 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A10
IHC of paraffin-embedded Human tonsil using anti-USP10 mouse monoclonal antibody.

Anti-USP10 Antibody (clone 2E1) - LS-C173493 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E1
IHC of paraffin-embedded Human prostate tissue using anti-USP10 mouse monoclonal antibody.

Anti-USP10 Antibody (N-Terminus) IHC-plus™ - LS-B5278 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-USP10 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5278 concentration 5 ug/ml.

Anti-USP10 Antibody (aa50-100) - LS-C93287 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-USP10 Antibody - LS-C191378 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-USP10 Antibody (aa500-529, AP) - LS-C273151 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP10 Antibody (aa500-529, APC) - LS-C273152 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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