RBBP4

Gene Symbol RBBP4
Entrez Gene 5928
Alt Symbol NURF55, RBAP48, lin-53
Species Human
Gene Type protein-coding
Description retinoblastoma binding protein 4
Other Description CAF-1 subunit C|CAF-I 48 kDa subunit|CAF-I p48|MSI1 protein homolog|RBBP-4|chromatin assembly factor 1 subunit C|chromatin assembly factor I p48 subunit|chromatin assembly factor/CAF-1 p48 subunit|histone-binding protein RBBP4|nucleosome-remodeling factor subunit RBAP48|retinoblastoma-binding protein 4|retinoblastoma-binding protein p48
Swissprots Q53H02 D3DPQ3 P31149 Q09028 Q96BV9 B4DRH0 B2R6G9
Accessions CAH61602 EAX07513 EAX07514 EAX07515 Q09028 AK056356 AK056550 AK222779 BAD96499 AK299251 BAG61282 AK299410 BAG61392 AK312571 BAG35466 BC003092 AAH03092 BC015123 AAH15123 BC053904 AAH53904 BC075836 AAH75836 BT007309 AAP35973 DC315298 DQ892499 ABM83425 DQ895712 ABM86638 X71810 CAA50685 X74262 CAA52321 NM_001135255 NP_001128727 NM_001135256 NP_001128728 NM_005610 NP_005601
Function Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. {ECO:0000269|PubMed:10866654}.
Subcellular Location Nucleus.

RBAP46/RBAP48 (D4F8) Rabbit mAb - 9067 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4F8
Western blot analysis of extracts from various cell lines using RBAP46/RBAP48 (D4F8) Rabbit mAb.

RBAP46/RBAP48 Antibody - 4633 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using RBAP46/RBAP48 Antibody.

RbAp46/p48 (G-8) - sc-373873 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RbAp46/p48 (G-8): sc-373873. Western blot analysis of RbAp46/p48 expression in HeLa nuclear extract.

RbAp46/p48 (H-300) - sc-33170 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RbAp46/p48 (H-300): sc-33170. Western blot analysis of RbAp46/p48 expression in HL-60 nuclear extract.

RbAp48 (K-15) - sc-12434 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RbAp48 (K-15): sc-12434. Western blot analysis of RbAp48 expression in Y79 (A), HeLa (B) and K-562 (C) nuclear extracts and Y79 (D) whole cell lysate.

RbAp48 (N-19) - sc-8270 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RbAp48 (N-19): sc-8270. Western blot analysis of RbAp48 expression in non-transfected: sc-117752 (A) and human RbAp48 transfected: sc-116563 (B) 293T whole cell lysates.

Anti-RbAp46+RbAp48 antibody - ab50307 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RbAp48 antibody - ab180647 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling RbAp48 with ab180647 at 1/100. Magnification: 200x.

Anti-RbAp48 antibody - ab55778 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
RbAp48 antibody (ab55778) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human transitional cell carcinoma tissue.

Anti-RbAp48 antibody [11G10] - ab488 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 11G10
Anti-RbAp48 antibody [11G10] (ab488) at 2 µg/ml + Whole cell lysates prepared from human colon adenocarcinoma SW480 cell line at 30 µgSecondaryHRP-conjugated goat anti-mouse IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-RbAp48 antibody [2D7] - ab117746 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2D7
Anti-RbAp48 antibody [2D7] (ab117746) + HeLa cell lysate at 500 µg/ml

Anti-RbAp48 antibody [EPR3411] - ab79416 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3411
All lanes : Anti-RbAp48 antibody [EPR3411] (ab79416) at 1/50000 dilutionLane 1 : HeLa lysateLane 2 : Jurkat lysateLane 3 : NIIH/3T3 lysateLane 4 : 293 lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-RbAp48 antibody [EPR3411] (Alexa Fluor® 488) - ab199816 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3411
ab199816 staining RbAp48 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-RbAp48 antibody [EPR3411] (Alexa Fluor® 647) - ab199817 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3411
ab199817 staining RbAp48 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Anti-RbAp48 antibody [EPR3412] - ab92344 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3412
All lanes : Anti-RbAp48 antibody [EPR3412] (ab92344) at 1/20000 dilutionLane 1 : HeLa lysateLane 2 : Jurkat cell lysateLane 3 : Raji cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-RBBP4 / RBAP48 Antibody (aa1-425, clone 15G12) - LS-C20043 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 15G12

Anti-RBBP4 / RBAP48 Antibody (aa1-425, clone 4H9) - LS-C53330 from LifeSpan Bioscience

Prices $445.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4H9

Anti-RBBP4 / RBAP48 Antibody (aa1-425, clone 1D11) - LS-C83415 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D11

Anti-RBBP4 / RBAP48 Antibody (aa1-425, clone 4H9) - LS-C53331 from LifeSpan Bioscience

Prices $445.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4H9

Anti-RBBP4 / RBAP48 Antibody (aa1-425, clone 3-255) - LS-C83416 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3-255

Anti-RBBP4 / RBAP48 Antibody (aa1-50) - LS-C93688 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-RBBP4 / RBAP48 Antibody - LS-C192890 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of various cell lines, using RBBP4 antibody.

Anti-RBBP4 / RBAP48 Antibody - LS-C179202 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RBBP4 / RBAP48 Antibody - LS-C305937 from LifeSpan Bioscience

Prices $560.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of RBBP4 / RBAP48 antibody LS-X223191.

Anti-RBBP4 / RBAP48 Antibody (C-Terminus) - LS-C170255 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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