Anti-RbAp48 antibody [11G10]
| Name | Anti-RbAp48 antibody [11G10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab488 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 11G10 |
| Applications | FC WB IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Fusion protein containing the complete coding region (amino acids 1-425) of RbAp48 expressed in E |
| Description | Mouse Monoclonal |
| Gene | RBBP4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-RbAp48 antibody [11G10] (ab488) at 2 µg/ml + Whole cell lysates prepared from human colon adenocarcinoma SW480 cell line at 30 µgSecondaryHRP-conjugated goat anti-mouse IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/21235_ab488-11996_copy.jpg)
Anti-RbAp48 antibody [11G10] (ab488) at 2 µg/ml + Whole cell lysates prepared from human colon adenocarcinoma SW480 cell line at 30 µgSecondaryHRP-conjugated goat anti-mouse IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing HeLa cells stained with ab488 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab488, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/21237_RbAp48-Primary-antibodies-ab488-2.jpg)
Overlay histogram showing HeLa cells stained with ab488 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab488, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
NIPP1 maintains EZH2 phosphorylation and promoter occupancy at - NIPP1 maintains EZH2 phosphorylation and promoter occupancy at
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2-D structure of the A region of Xist RNA and its implication for PRC2 - 2-D structure of the A region of Xist RNA and its implication for PRC2
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The phosphatase interactor NIPP1 regulates the occupancy of the histone - The phosphatase interactor NIPP1 regulates the occupancy of the histone
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Ageing-related chromatin defects through loss of the NURD complex. - Ageing-related chromatin defects through loss of the NURD complex.
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A Lin-9 complex is recruited by B-Myb to activate transcription of G2/M genes in - A Lin-9 complex is recruited by B-Myb to activate transcription of G2/M genes in
Knight AS, Notaridou M, Watson RJ. Oncogene. 2009 Apr 16;28(15):1737-47.
Atypical Mowat-Wilson patient confirms the importance of the novel association - Atypical Mowat-Wilson patient confirms the importance of the novel association
Verstappen G, van Grunsven LA, Michiels C, Van de Putte T, Souopgui J, Van Damme J, Bellefroid E, Vandekerckhove J, Huylebroeck D. Hum Mol Genet. 2008 Apr 15;17(8):1175-83.