THEMIS

Gene Symbol	THEMIS
Entrez Gene	387357
Alt Symbol	C6orf190, C6orf207, GASP, SPOT, TSEPA, bA325O24.3, bA325O24.4
Species	Human
Gene Type	protein-coding
Description	thymocyte selection associated
Other Description	GRB2-associated protein\|protein THEMIS\|signaling phosphoprotein specific for T cells\|thymocyte expressed molecule involved in selection\|thymocyte selection pathway associated\|thymocyte-expressed molecule involved in selection
Swissprots	Q5T3C4 Q8N1K5 Q6MZT7 F5H1J9 A1L4F0 B3KY07 Q5T3C5 A8K7N1 B3KT31 B3KW32
Accessions	CAY56007 CBX47634 EAW48091 EAW48092 Q8N1K5 AK094863 BAG52943 AK097903 BAC05194 AK124031 BAG53994 AK128377 BAG54669 AK292046 BAF84735 BC043608 BC130516 AAI30517 BC144034 BG461112 BX640890 CAE45941 BX956657 DA818250 HQ258481 ADR83235 XM_011535813 XP_011534115 XM_011535814 XP_011534116 XM_011535815 XP_011534117 XM_011535816 XP_011534118 XM_011535817 XP_011534119 XM_011546483 XP_011544785 XM_011546484 XP_011544786 XM_011546485 XP_011544787 XM_011546486 XP_011544788 XM_011546487 XP_011544789 NM_001010923 NP_001010923 NM_001164685 NP_001158157 NM_001164687 NP_001158159
Function	Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk (By similarity). {ECO:0000250}.
Subcellular Location	Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.

THEMIS Antibody - 4482 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from various cell lines and human primary T cells using THEMIS Antibody.

Themis (A-11) - sc-514012 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Themis (A-11): sc-514012. Western blot analysis of Themis expression in Jurkat (A) and MOLT-4 (B) whole cell lysates, Jurkat nuclear extract (C) and human thymus tissue extract (D).

Themis (C-19) - sc-244309 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Themis (C-19): sc-244309. Western blot analysis of Themis expression in Jurkat (A) and MOLT-4 (B) whole cell lysates.

Themis (N-19) - sc-244307 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Themis (N-19): sc-244307. Western blot analysis of Themis expression in Jurkat whole cell lysate (A) and mouse spleen (B) and mouse thymus (C) tissue extracts.

Anti-Themis antibody - ab128272 from Abcam

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Themis antibody (ab128272) at 1 µg/ml + Jurkat cell lysate at 10 µg

Anti-Themis antibody [EPR7353] - ab126771 from Abcam

Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7353

All lanes : Anti-Themis antibody [EPR7353] (ab126771) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : Human thymus lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-Themis antibody [EPR7354] - ab129174 from Abcam

Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7354

All lanes : Anti-Themis antibody [EPR7354] (ab129174) at 1/10000 dilutionLane 1 : Human thymus lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Themis antibody - N-terminal - ab157811 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Lanes 1 & 5 : Anti-Themis antibody - N-terminal (ab157811) at 1/2000 dilutionLanes 2 & 6 : Anti-Themis antibody - N-terminal (ab157811) at 1/1000 dilutionLanes 3, 4, 7 & 8 : Anti-Themis antibody - N-terminal (ab157811) at 1/500 dilutionLane 1 : Mouse spleen tissue lysateLane 2 : Mouse spleen tissue lysateLane 3 : Mouse spleen tissue lysateLane 4 : Mouse spleen tissue lysate with blocking peptideLane 5 : K562 cell lysateLane 6 : K562 cell lysateLane 7 : K562 cell lysateLane 8 : K562 cell lysate with blocking peptide

Themis Antibody - NBP1-90802 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Themis Antibody [NBP1-90802] - Analysis in human cell line Jurkat.

Themis Antibody (701010) - MAB6929 from Novus Biologicals

Prices	$99.00, $249.00
Sizes	25 µg, 100 µg
Host	Mouse
Clonality	Monoclonal
Clone	701010

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Themis Monoclonal Antibody (Catalog # MAB6929) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Themis Antibody - NBP2-38183 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry: Themis Antibody [NBP2-38183] - Staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells while weak staining in a subset of germinal center cells.

THEMIS Polyclonal Antibody - bs-12329R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin embedded Rat lung spleen labeled with Anti-THEMIS Polyclonal Antibody, Unconjugated (bs-12329R) at 1:300, followed by conjugation to the secondary antibody and DAB staining.

THEMIS Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-12329R-A350 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-12329R-A488 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-12329R-A555 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated - bs-12329R-A647 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, Cy3 Conjugated - bs-12329R-Cy3 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, Cy5 Conjugated - bs-12329R-Cy5 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, Cy5.5 Conjugated - bs-12329R-Cy5.5 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, Cy7 Conjugated - bs-12329R-Cy7 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, FITC Conjugated - bs-12329R-FITC from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, Biotin Conjugated - bs-12329R-Biotin from Bioss

Prices	$349.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

THEMIS Polyclonal Antibody, HRP Conjugated - bs-12329R-HRP from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ANTI-THEMIS (C-TERM) antibody produced in rabbit - SAB1303221 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>THEMIS (C-term): <B>Cat. No. SAB1303221</B>: THEMIS in Human thymoma tissue with THEMIS antibody (C-term) at 12.5 μg/mL.

Anti-THEMIS antibody produced in rabbit - HPA031425 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-THEMIS: <B>Cat. No. HPA031425</B>: Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Search more