
Lanes 1 & 5 : Anti-Themis antibody - N-terminal (ab157811) at 1/2000 dilutionLanes 2 & 6 : Anti-Themis antibody - N-terminal (ab157811) at 1/1000 dilutionLanes 3, 4, 7 & 8 : Anti-Themis antibody - N-terminal (ab157811) at 1/500 dilutionLane 1 : Mouse spleen tissue lysateLane 2 : Mouse spleen tissue lysateLane 3 : Mouse spleen tissue lysateLane 4 : Mouse spleen tissue lysate with blocking peptideLane 5 : K562 cell lysateLane 6 : K562 cell lysateLane 7 : K562 cell lysateLane 8 : K562 cell lysate with blocking peptide