CD276

Gene Symbol CD276
Entrez Gene 80381
Alt Symbol 4Ig-B7-H3, B7-H3, B7H3, B7RP-2
Species Human
Gene Type protein-coding
Description CD276 molecule
Other Description B7 homolog 3|CD276 antigen|costimulatory molecule
Swissprots Q8NCB6 Q6P5Y4 Q8NBI8 Q5ZPR3 Q9BXR1 Q8NC34 Q6UXI2
Accessions CAC18949 CAC35428 EAW77933 EAW77934 Q5ZPR3 AF302102 AAK15438 AJ583695 CAE47548 AJ583696 AK074849 BAC11243 AK074997 BAC11344 AK075549 BAC11692 AK291721 BAF84410 AK292711 BAF85400 AK296358 BAG59038 AK298522 BAG60725 AK309728 AL360136 AY358343 AAQ88709 BC011578 BC062581 AAH62581 BQ278722 CA420873 CB529187 XM_005254700 XP_005254757 XM_011522095 XP_011520397 XM_011522096 XP_011520398 NM_001024736 NP_001019907 NM_025240 NP_079516
Function May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. {ECO:0000269|PubMed:11224528, ECO:0000269|PubMed:12906861, ECO:0000269|PubMed:14764704, ECO:0000269|PubMed:15314238, ECO:0000269|PubMed:15682454, ECO:0000269|PubMed:15961727}.
Subcellular Location Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.
Tissue Specificity Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. {ECO:0000269|PubMed:11224528, ECO:0000269|PubMed:14764704, ECO:0000269|PubMed:15961727, ECO:0000269|PubMed:16049332, ECO:0000269|PubMed:16274630}.
Top Pathways Cell adhesion molecules (CAMs)

B7-H3 (D9M2L) XP ® Rabbit mAb - 14058 from Cell Signaling Technology

Prices $109.00, $282.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9M2L
Western blot analysis of extracts from various cell lines using B7-H3 (D9M2L) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

B7-H3 (C-1) - sc-398323 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B7-H3 (C-1): sc-398323. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.

B7-H3 (D-8) - sc-398444 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B7-H3 (D-8): sc-398444. Western blot analysis of B7-H3 expression in A-431 (A), JAR (B) and HUV-EC-C (C) whole cell lysates.

B7-H3 (F-11) - sc-376769 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B7-H3 (F-11): sc-376769. Western blot analysis of B7-H3 expression in JAR (A) and JEG-3 (B) whole cell lysates.

B7-H3 (G-8) - sc-376535 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B7-H3 (G-8): sc-376535. Western blot analysis of B7-H3 expression in JAR (A) and JEG-3 (B) whole cell lysates.

B7-H3 (G-12) - sc-398403 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B7-H3 (G-12): sc-398403. Western blot analysis of B7-H3 expression in JAR (A) and HUV-EC-C (B) whole cell lysates.

B7-H3 (D-15) - sc-34569 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
B7-H3 (D-15): sc-34569. Western blot analysis of B7-H3 expression in A-431 (A), PMA treated Jurkat (B), JAR (C), Neuro-2a (D) and HUV-EC-C (E) whole cell lysates.

B7-H3 (H-300) - sc-66814 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
B7-H3 (H-300): sc-66814. Western blot analysis of mouse recombinant B7-H3.

Anti-CD276 antibody - ab105434 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CD276 antibody (ab105434) at 1 µg/mlLane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lane 2 : Cervix (Human) Whole Cell Lysate - adult normal tissue (ab29226)Lane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-CD276 antibody - ab128386 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CD276 antibody (ab128386) at 1 µg/ml + MCF7 cell lysate at 10 µg

Anti-CD276 antibody [6A1] - ab105922 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6A1
All lanes : Anti-CD276 antibody [6A1] (ab105922) at 1/200 dilutionLane 1 : HEK293 cell lysateLane 2 : Transfected HEK293 cell lysate

Anti-CD276 antibody [EPNCIR122] - ab134161 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR122
Overlay histogram showing THP1 cells stained with ab134161 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134161, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CD276 antibody [M3.2D7] - Low endotoxin, Azide free - ab185777 from Abcam

Prices $376.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone M3.2D7
Flow cytometric analysis of non-transfected (left) and mouse CD276 transfected (right) 293T cells with 0.25 µg of Rat IgG2a Isotype Control (open histogram) or 0.25 µg of ab185777 (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total cells were used for analysis.

Anti-CD276 antibody [MIH42] (Phycoerythrin) - ab110224 from Abcam

Prices $188.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone MIH42
ab110224 staining CD276 in peripheral human blood monocytes by Flow cytometry.

Anti-CD276 antibody [MM0104-20J12] - ab89133 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone MM0104-20J12
IHC image of ab89133 staining in Human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89133, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD276 antibody - C-terminal - ab191161 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-CD276 antibody - C-terminal (ab191161) at 1/1000 dilution + MCF7 cell lysate at 35 µg

Anti-CD276 antibody - C-terminal - ab171197 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CD276 antibody - C-terminal (ab171197) at 1/100 dilution + MCF7 cell lysate at 35 µg

Anti-CD276 / B7-H3 Antibody (clone IAH:F799:IC12) - LS-C58390 from LifeSpan Bioscience

Prices $375.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal
Clone IAH:F799:IC12

Anti-CD276 / B7-H3 Antibody (PE) - LS-C74208 from LifeSpan Bioscience

Prices $455.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal

Anti-CD276 / B7-H3 Antibody - LS-C78908 from LifeSpan Bioscience

Prices $485.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal

Anti-CD276 / B7-H3 Antibody (clone MIH42, RPE) - LS-C58218 from LifeSpan Bioscience

Prices $350.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal
Clone MIH42
Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD276: RPE.

Anti-CD276 / B7-H3 Antibody (clone MIH42, FITC) - LS-C58216 from LifeSpan Bioscience

Prices $330.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MIH42
Peripheral Human monocytes stained with Mouse anti Human CD276: FITC.

Anti-CD276 / B7-H3 Antibody - LS-C128035 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CD276 / B7-H3 Antibody (clone MIH42) - LS-C123197 from LifeSpan Bioscience

Prices $530.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone MIH42

Anti-CD276 / B7-H3 Antibody (FITC) - LS-C128036 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Search more