CD86

Gene Symbol CD86
Entrez Gene 942
Alt Symbol B7-2, B7.2, B70, CD28LG2, LAB72
Species Human
Gene Type protein-coding
Description CD86 molecule
Other Description B-lymphocyte activation antigen B7-2|BU63|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)|CTLA-4 counter-receptor B7.2|FUN-1|T-lymphocyte activation antigen CD86
Swissprots E7ETN5 Q7M4L5 Q6GTS4 E9PC27 B7Z702 A0N0P0 B7Z2F3 P42081 Q13655 Q6FHB1
Accessions AAA86473 ABK41931 CDM22279 EAW79493 EAW79494 P42081 AK291497 BAF84186 AK294663 BAH11839 AK301237 BAH13438 AK313103 BAG35926 AK316203 BAH14574 BC040261 AAH40261 BX093060 CD705810 CR541844 CAG46642 DA619360 DQ890617 ABM92234 DQ893787 ABM84713 L25259 AAA58389 U04343 AAB03814 NM_001206924 NP_001193853 NM_001206925 NP_001193854 NM_006889 NP_008820 NM_175862 NP_787058 NM_176892 NP_795711
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Subcellular Location Cell membrane; Single-pass type I membrane protein.
Tissue Specificity Expressed by activated B-lymphocytes and monocytes.
Top Pathways Type I diabetes mellitus, Graft-versus-host disease, Allograft rejection, Autoimmune thyroid disease, Intestinal immune network for IgA production

B7-2 (C-19) - sc-1635 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
B7-2 (C-19): sc-1635. Western blot analysis of B7-2 expression in Raji whole cell lysate.

B7-2 (N-18) - sc-1636 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
B7-2 (N-18): sc-1636. Western blot analysis of B7-2 expression in Jurkat whole cell lysate.

B7-2 (H-200) - sc-9092 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
B7-2 (H-200): sc-9092. Western blot analysis of B7-2 expression in non-transfected 293T: sc-117752 (A), human B7-2 transfected 293T: sc-175289 (B) and Raji (C) whole cell lysates.

B7-2 (D-6) - sc-28347 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B7-2 (D-6): sc-28347. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic and membrane staining of non follicle cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

B7-2 (BU63) - sc-19617 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B7-2 (BU63) PE: sc-19617 PE. FCM analysis of human peripheral blood leukocytes. Quadrant markers were set based on the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

B7-2 (B-T7) - sc-58915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
B7-2 (B-T7): sc-58915. Western blot analysis of B7-2 expression in Raji (A), BJAB (B), GA-10 (C) and NAMALWA (D) whole cell lysates.

Anti-CD86 antibody - ab79683 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CD86 antibody (ab79683) at 1 µg/mlLane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 6 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-CD86 antibody - ab137723 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CD86 antibody (ab137723) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-CD86 antibody [1B3] - ab130164 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B3
All lanes : Anti-CD86 antibody [1B3] (ab130164) at 1/500 dilutionLane 1 : L1210 cell lysatesLane 2 : MOLT4 cell lysates

Anti-CD86 antibody [B-T7] - ab27339 from Abcam

Prices $378.00
Sizes 1 ml
Host Mouse
Clonality Monoclonal
Clone B-T7
Ab27339, staining CD86 by FACS.

Anti-CD86 antibody [B-T7] - ab47170 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone B-T7
A typical staining pattern with ab47170 monoclonal antibody of monocytes

Anti-CD86 antibody [B-T7] (FITC) - ab27361 from Abcam

Prices $378.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone B-T7
A typical staining pattern using ab27361 of Monocytes.

Anti-CD86 antibody [B-T7] (Phycoerythrin) - ab27323 from Abcam

Prices $376.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone B-T7
Ab27323, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD86 by Flow Cytometry.

Anti-CD86 antibody [BU63] - ab23556 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BU63

Anti-CD86 antibody [BU63] (Alexa Fluor® 647) - ab187770 from Abcam

Prices $370.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone BU63

Anti-CD86 antibody [BU63] (FITC) - ab77276 from Abcam

Prices $188.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone BU63

Anti-CD86 antibody [BU63] (PerCP) - ab77131 from Abcam

Prices $188.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone BU63
ab77131 staining, surface CD86 in human peripheral blood cells by Flow Cytometery. Monocytes were used for gating. The blue peak reperesents negative control while red peak show staining with ab77131.

Anti-CD86 antibody [BU63] (Phycoerythrin) - ab77226 from Abcam

Prices $191.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone BU63
Flow cytometry analysis of human peripheral blood leukocytes with anti-human CD86 (BU63) PE.

Anti-CD86 antibody [BU63], prediluted (Allophycocyanin) - ab134385 from Abcam

Prices $378.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone BU63
ab134385 staining CD86 in Human PBMCs by Flow Cytometry. The sample was incubated with the primary antibody (1/50 in 2% Human serum + 0.5mM EDTA in PBS) for 25 minutes at 4°C.Gating Strategy: Lymphocytes. See Abreview

Anti-CD86 antibody [EP1158Y] - ab53004 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1158Y
ab53004 staining CD86 in Cynomolgus Monkey lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/800) for 30 minutes at room temperature. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

Anti-CD86 antibody [EP1158Y] (HRP) - ab196916 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1158Y
IHC image of CD86 staining in a section of formalin-fixed paraffin-embedded normal human spleen tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196916 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Anti-CD86 antibody [IT2.2] (Biotin) - ab95746 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone IT2.2

Anti-CD86 antibody [IT2.2] (PE/Cy5®) - ab95745 from Abcam

Prices $191.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone IT2.2
Staining of normal Human peripheral blood cells with Mouse IgG2b Isotype Control PE-Cy5 (blue histogram) or ab95745 (purple histogram). Cells in the monocyte gate were used for analysis.

Anti-CD86 antibody [IT2.2] - Low endotoxin, Azide free - ab185774 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone IT2.2

Anti-CD86 antibody - N-terminal - ab196564 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoflourescent analysis of MCF-7 cells labeling CD86 with ab196564 at 1/50 dilution. Blue: DAPI for nuclear staining.
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