All lanes : Anti-CD86 antibody (ab79683) at 1 µg/mlLane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 6 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.