HIST2H2BD

Gene Symbol HIST2H2BD
Entrez Gene 337874
Alt Symbol H2B/o, H2B/s, H2BFO
Species Human
Gene Type pseudo
Description histone cluster 2, H2bd (pseudogene)
Other Description H2B histone family, member O|histone 2, H2bd
Swissprots Q6DRA6
Accessions Q6DRA6 BC068044
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Location Nucleus. Chromosome.

Anti-Histone H2B antibody - ab45695 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of ab45695 staining Histone H2B in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45695, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset). For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H2B antibody - ab134211 from Abcam

Prices $384.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
ICC/IF image of ab134211 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab134211 at 0.1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken IgY (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Histone H2B antibody - ab18977 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H2B antibody (ab18977) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Observed band size : 14 kDa (why is the actual band size different from the predicted?)

Anti-Histone H2B antibody - ab61250 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61250 at 1/500 - 1/1000 dilution staining Histone H2B in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-Histone H2B antibody - ab61245 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61245 staining Histone H2B in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab61245 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410), which was added to the secondary antibody mixture. A secondary only negative control is displayed, which indicates that the Histone H2B staining observed is due to primary antibody specificity and not to unspecific binding of the secondary antibody to the cells.

Anti-Histone H2B antibody - ab104743 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2B antibody (ab104743) at 1/500 dilutionLane 1 : HeLa cell line lysateLane 2 : 293 cell line lysate

Anti-Histone H2B antibody - ab63637 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2B antibody (ab63637) at 1/500 dilutionLane 1 : Jurkat cell extracts treated with UV (15mins)Lane 2 : Jurkat cell extracts treated with UV (15mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-Histone H2B antibody - ab156197 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Histone H2B antibody (ab156197) at 1/100 dilution + WiDR cell lysate at 35 µg

Anti-Histone H2B antibody [EP819Y] - ChIP Grade - ab52985 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP819Y
IHC image of ab52985 staining Histone H2B in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52985, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset). For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H2B antibody [EP957Y] - ab52599 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP957Y
Overlay histogram showing HeLa cells stained with ab52599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52599, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Histone H2B antibody [EP957Y] (HRP) - ab200072 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP957Y
All lanes : Anti-Histone H2B antibody [EP957Y] (HRP) (ab200072) at 1/5000 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 3 : Histone H3 Recombinant protein at 0.1 µgLane 4 : Histone 2B Recombinant protein at 0.1 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H2B antibody (FITC) - ab192938 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Histone H2B antibody (HRP) - ab64039 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2B antibody (HRP) (ab64039) at 1/40000 dilutionLane 1 : Histone H1 Recombinant ProteinLane 2 : Histone H2A Recombinant ProteinLane 3 : Histone H2B Recombinant ProteinLane 4 : Histone H3.1 Recombinant ProteinLane 5 : Histone H4 Recombinant ProteinLysates/proteins at 0.1 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade - ab52484 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 52484
ab52484 staining Histone H2B in Fruit fly (Drosophila melanogaster) embryo cells by ICC/IF (Immunocytochemistry/immunofluorescence). Embryos were washed in 1x PBS + 0.1% Trition, cells were fixed with heat, and blocked with 10% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/1000) for 24 hours. An Alexa Fluor®594-conjugated Rabbit anti-mouse IgG polyclonal (1/5000) was used as the secondary antibody.See Abreview

Anti-Histone H2B antibody [mAbcam 64165] (Alexa Fluor® 647) - ab202576 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam 64165
ab202576 staining Histone H2B in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202576 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Histone H2B antibody [mAbcam 64165] - ChIP Grade - ab64165 from Abcam

Prices $397.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 64165
Histone H2B was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Histone H2B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64165.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 17kDa; Histone H2B

Anti-Histone H2B antibody - ChIP Grade - ab1790 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
developed using the ECL techniquePerformed under reducing conditions.

Anti-Histone H2B antibody - N-terminal - ab139383 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of formalin-fixed, paraffin-embedded Human tonsil tissue labelling Histone H2B with ab139383 at 10&microg/ml.
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