Array



Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody - ab14723 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody (ab14723) at 1/1000 dilutionLane 1 : YDH8 cka 2-8 ts grown at 30 degrees CLane 2 : YDH8 cka 2-8 ts grown at 38 degrees CSecondaryHRP conjugated anti-Rabbit

Anti-Histone H2A (phospho S129) antibody - ChIP Grade - ab15083 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All batches of ab15083 are tested in Peptide Array against peptides to different Histone H2A modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H2A - phospho S129 (ab19828), indicating that this antibody specifically recognises the Histone H2A - phospho S129 modification.ab19828 - Histone H2A - phospho S129ab19751 - Histone H2A - unmodified

Anti-Histone H2A (symmetric di methyl R29) antibody - ab129208 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H2A (symmetric di methyl R29) antibody (ab129208) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H2A.X (acetyl K5) antibody - ab129217 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab129217 stained HeLa cells. The cells were exposed to 2000µJ/cm2 of UV, incubated at 37˚C for a further 24 hours. The cells were then 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab129217, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Histone H2B (glcnac S112) antibody - ab130951 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All batches of ab130951 are tested in Peptide Array against peptides to different Histone H2B modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H2B - GlcNAc S112 peptide (ab166685), indicating that this antibody recognises the Histone H2B - GlcNAc S112 modification. This product shows <20% cross reactivity in peptide array with the unmodified peptide and does not cross react with GlcNAc S36, GlcNAc T101 or GlcNAc S47.ab166685 - Histone H2B - GlcNAc S112ab166686 - Histone H2B - unmodifiedab166688 - Histone H2B - GlcNAc S36

Anti-Histone H3 (acetyl K18) antibody - ChIP Grade - ab1191 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All batches of ab1191 are tested in Peptide Array against peptides to different Histone H3 and H4 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - acetyl K18 peptide (ab24003), indicating that this antibody specifically recognises the Histone H3 - acetyl K18 modification.ab17163 - Histone H3 - unmodifiedab16635 - Histone H3 - acetyl K9ab15591 - Histone H3 - acetyl K14ab24003 - Histone H3 - acetyl K18ab48359 - Histone H3 - acetyl K23ab24404 - Histone H3 - acetyl K27ab41409 - Histone H3 - acetyl K36ab15662 - Histone H4 - acetyl K12

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade - ab4729 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab4729 staining Histone H3 (acetyl K27) in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4729, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Histone H3 (asymmetric di methyl R17) antibody - ChIP Grade - ab8284 from Abcam

Prices $403.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All batches of ab8284 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - asymmetric di methyl R17 peptide (ab16935), indicating that this antibody specifically recognises the Histone H3 - asymmetric di methyl R17 modification.ab16935 - Histone H3 - asymmetric di methyl R17ab32948 - Histone H3 - symmetric di methyl R17ab14663 - Histone H3 - unmodified

Anti-Histone H3 (asymmetric di methyl R17, symmetric di methyl R17) antibody - ab174566 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All batches of ab174566 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - asymmetric di methyl R17 peptide (ab16935) and Histone H3 - symmetric di methyl R17 peptide (ab32948).ab14663 - Histone H3 - unmodifiedab16935 - Histone H3 - asymmetric di methyl R71ab32948 - Histone H3 - symmetric di methyl R17

Anti-Histone H3 (asymmetric di methyl R8) antibody - ab127163 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab127163 staining Histone H3 (asymmetric di methyl R8) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab127163, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade - ab5103 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade (ab5103) at 1/1000 dilution + Fruit fly (Drosophila melanogaster) tissue lysate - nuclear at 5 µgSecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (di methyl K4) antibody - ChIP Grade - ab7766 from Abcam

Prices $403.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab7766 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Anti-Histone H3 (di methyl K79) antibody - ChIP Grade - ab3594 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab3594 staining Histone H3 (di methyl K79) in human differentiated haematopoietic stem cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/800) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated goat polyclonal anti-rabbit IgG (1/1500) was used as the secondary antibody.Gating Strategy: Isotype negative control (white).See Abreview

Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade - ab2886 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab2886 staining Histone H3 (mono methyl K79) in human differentiated haematopoietic stem cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/800) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat polyclonal anti-rabbit IgG was used as the secondary antibody.Gating Strategy: Isotype negative control (white).See Abreview

Anti-Histone H3 (mono methyl R17) antibody - ab130283 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
IHC image of ab47503 staining Histone H3 (mono methyl R17) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab130283, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 (mono methyl R2) antibody - ChIP Grade - ab15584 from Abcam

Prices $401.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 2µg of  ab15584 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.     

Anti-Histone H3 (mono methyl R26) antibody - ab130898 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 (mono methyl R26) antibody (ab130898) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (phospho S28) antibody - ab5169 from Abcam

Prices $401.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 (phospho S28) antibody (ab5169) at 1 µg/ml + Hela Whole Cell Lysate - Colcemid Treated at 2.5 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (phospho T3) antibody - ab130940 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab130940 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab130940, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488  (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

Anti-Histone H3 (phospho T6) antibody - ChIP Grade - ab14102 from Abcam

Prices $400.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 (phospho T6) antibody - ChIP Grade (ab14102) at 1 µg + HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (symmetric di methyl R26) antibody - ab127095 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 (symmetric di methyl R26) antibody (ab127095) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (symmetric di methyl R8) antibody - ab130740 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab130740 staining Histone H3 (symmetric di methyl R8) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab130740, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade - ab108245 from Abcam

Host Rabbit
Clonality Polyclonal
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab108245 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade - ab8580 from Abcam

Prices $404.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab8580 staining Histone H3 (tri methyl K4) in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8580, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Histone H4 (acetyl K5) antibody - ab114146 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H4 (acetyl K5) antibody (ab114146) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
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