All batches of ab130898 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - mono methyl R26 peptide (ab154426), indicating that this antibody specifically recognises the Histone H3 - mono methyl R26 modification.ab154426 - Histone H3 - mono methyl R26ab154427 - Histone H3 - symmetric di methyl R26ab2854 - Histone H3 - asymmetric di methyl R26ab17163 - Histone H3 - unmodifiedab154424 - Histone H3 - mono methyl R17ab154425 - Histone H3 - symmetric di methyl R17ab16935 - Histone H3 - asymmetric di methyl R17

ICC/IF image of ab130898 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab130898 at 1µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.