All batches of ab114146 are tested in Peptide Array against peptides to different Histone H4 and H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - acetyl K5 peptide (ab154430), indicating that this antibody specifically recognises the Histone H4 - acetyl K5 modification.ab154430 - Histone H4 - acetyl K5ab154467 - Histone H4 - unmodifiedab15824 - Histone H4 - acetyl K8ab154463 - Histone H4 - acetyl K12ab154464 - Histone H4 - acetyl K16ab20632 - Histone H4 - acetyl K20ab16635 - Histone H3 - acetyl K9

ICC/IF image of ab114146 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab114146 at 0.1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.