Gene Symbol | APLF |
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Entrez Gene | 200558 |
Alt Symbol | APFL, C2orf13, PALF, Xip1, ZCCHH1 |
Species | Human |
Gene Type | protein-coding |
Description | aprataxin and PNKP like factor |
Other Description | PNK and APTX-like FHA domain-containing protein|PNK and APTX-like FHA protein|XRCC1-interacting protein 1|aprataxin and PNK-like factor|aprataxin- and PNK-like factor|apurinic-apyrimidinic endonuclease APLF|zinc finger, CX5CX6HX5H motif containing 1 |
Swissprots | Q53P47 Q53PB9 A8K476 Q8IW19 Q53QU0 |
Accessions | AAY14945 AAY24008 AAY24113 EAW99871 EAW99872 Q8IW19 AK290841 BAF83530 AK308186 BC030711 BC041144 AAH41144 DA641644 NM_173545 NP_775816 |
Function | Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic- apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. {ECO:0000269|PubMed:17353262, ECO:0000269|PubMed:17396150}. |
Subcellular Location | Nucleus. Cytoplasm, cytosol. Note=Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers. |
APLF CRISPR/Cas9 KO Plasmid (h) - sc-405415 from Santa Cruz Biotechnology
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APLF HDR Plasmid (h) - sc-405415-HDR from Santa Cruz Biotechnology
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APLF Double Nickase Plasmid (h) - sc-405415-NIC from Santa Cruz Biotechnology
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APLF Double Nickase Plasmid (h2) - sc-405415-NIC-2 from Santa Cruz Biotechnology
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APLF CRISPR Activation Plasmid (h) - sc-405415-ACT from Santa Cruz Biotechnology
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APLF CRISPR Activation Plasmid (h2) - sc-405415-ACT-2 from Santa Cruz Biotechnology
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APLF Lentiviral Activation Particles (h) - sc-405415-LAC from Santa Cruz Biotechnology
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APLF Lentiviral Activation Particles (h2) - sc-405415-LAC-2 from Santa Cruz Biotechnology
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APLF siRNA (h) - sc-94862 from Santa Cruz Biotechnology
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APLF shRNA Plasmid (h) - sc-94862-SH from Santa Cruz Biotechnology
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APLF shRNA (h) Lentiviral Particles - sc-94862-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU107491 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3322 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3322 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR11068 from Sigma-Aldrich
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APLF siRNA (Human) - CRJ6255 from Cohesion Biosciences
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APLF - MBS8204868 from MyBioSource
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shRNA set against Human APLF (NM_173545.2) - SHH238258 from Creative biogene
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Human APLF siRNA - orb276756 from Biorbyt
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APLF shRNA Plasmid (Human) - CHJ6255 from Cohesion Biosciences
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APLF miRNA 3'UTR clone - MiUTR4H-TG00406 from Creative biogene
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