IKBKG

Gene Symbol IKBKG
Entrez Gene 8517
Alt Symbol AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
Species Human
Gene Type protein-coding
Description inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Other Description I-kappa-B kinase subunit gamma|IKKAP1|IKKG|IkB kinase gamma subunit|NF-kappa-B essential modifier|NF-kappa-B essential modulator|NFkappaB essential modulator|ikB kinase subunit gamma|ikB kinase-associated protein 1|incontinentia pigmenti|inhibitor of nuclear factor kappa-B kinase subunit gamma
Swissprots Q7Z7F1 Q7LBY6 Q9Y6K9
Accessions AAL27012 CAB93146 EAW72677 EAW72678 EAW72679 EAW72680 EAW72681 Q9Y6K9 AF062089 AAD12183 AF074382 AAC36330 AF091453 AAD38081 AF261086 AAF99679 AK000593 AK308366 AY114157 AAM44073 AY429546 AY429547 AY429548 BC000299 AAH00299 BC012114 AAH12114 BC046922 AAH46922 BC050612 AAH50612 BT019621 AAV38427 DC410130 DQ891383 ABM82309 DQ894560 ABM85486 XM_005274760 XP_005274817 XM_005274761 XP_005274818 XM_005274764 XP_005274821 XM_011531203 XP_011529505 XM_011531204 XP_011529506 XM_011531205 XP_011529507 XM_011531206 XP_011529508 XM_011531207 XP_011529509 NM_001099856 NP_001093326 NM_001099857 NP_001093327 NM_001145255 NP_001138727 NM_003639 NP_003630
Function Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'- linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination. {ECO:0000269|PubMed:14695475, ECO:0000269|PubMed:19854139, ECO:0000269|PubMed:20724660}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:14651848}. Nucleus {ECO:0000269|PubMed:14651848}. Note=Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Top Pathways Herpes simplex infection, Viral carcinogenesis, PI3K-Akt signaling pathway, HTLV-I infection, Epstein-Barr virus infection

Phospho-IKKγ (Ser376) Antibody - 2689 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or treated with TNF-α (20 ng/ml) and Calyculin A #9902 (50 nM), using Phospho-IKKγ (Ser376) Antibody (upper) or IKKγ Antibody #2685 (lower).

IKKγ (DA10-12) Mouse mAb - 2695 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (20 western blots)
Host Mouse
Clonality Monoclonal
Clone DA10-12
Western blot analysis of extracts from HeLa cells, untreated or treated with TNF-α (20 ng/ml) and Calyculin A #9902 (50 nM), using Phospho-IKKγ (Ser376) Antibody #2689 (top) or IKKγ (DA10-12) Mouse mAb (bottom).

IKKγ Antibody - 2685 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using IKKγ Antibody.

IKKγ (B-10) - sc-166700 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IKKγ (B-10): sc-166700. Western blot analysis of IKKγ expression in non-transfected: sc-117752 (A) and human IKKγ transfected: sc-116282 (B) 293T whole cell lysates.

IKKγ (B-3) - sc-8032 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IKKγ (B-3): sc-8032. Western blot analysis of IKKγ expression in HL-60 whole cell lysate.

IKKγ (FL-419) - sc-8330 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
IKKγ (FL-419): sc-8330. Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of follicle and non-follicle cells and surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

IKKγ (M-18) - sc-8256 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
IKKγ (M-18): sc-8256. Western blot analysis of IKKγ expression in Jurkat (A) and HL-60 (B) whole cell lysates.

IKKγ (46B844) - sc-52930 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
IKKγ (46B844): sc-52930. Western blot analysis of IKKγ expression in non-transfected: sc-117752 (A) and human IKKγ transfected: sc-116282 (B) 293T whole cell lysates.

IKKγ (1.T.26) - sc-71331 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
IKKγ (1.T.26): sc-71331. Western blot analysis of IKKγ expression in BJAB (A), Jurkat (B), K-562 (C), U-937 (D), SW-13 (E) and HeLa (F) whole cell lysates.

IKKγ (72C627) - sc-56919 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
IKKγ (72C627): sc-56919. Western blot analysis of IKKγ expression in BJAB (A), Jurkat (B), K-562 (C), U-937 (D), SW-13 (E) and HeLa (F) whole cell lysates.

p-IKKγ (Ser 376) - sc-31721 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-IKK gamma [EPR16629] antibody - ab178872 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16629
Immunofluorescence analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells (Mouse embyro fibroblast cells) labeling IKK gamma (green) with ab178872 at 1/250 dilution showing cytoplasm and nucleus staining. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution. Counter stain is labeling tubulin (red) with ab7291 at 1/500 dilution with secondary antibody Goat anti-Mouse AlexaFluor® 594 (ab150120) at 1/400 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab178872 at 1/250 dilution, ab150120 at 1/400 dilution. -ve control 2 is ab7291 at 1/500 dilution, ab150077 at 1/200 dilution.

Anti-IKK gamma (phospho S31) antibody - ab55342 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IKK gamma (phospho S31) antibody (ab55342) at 1/500 dilutionLane 1 : Extracts from 293 cells treated with TNF alpha (20ng/ml for 5 mins).Lane 2 : Extracts from 293 cells treated with TNF alpha (20ng/ml for 5 mins), and with the immunising phosphopeptide.

Anti-IKK gamma (phospho S31) antibody - ab192212 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IKK gamma (phospho S31) antibody (ab192212) at 1/500 dilutionLane 1 : TNF-a treated 293 whole cell lysates with Antigen-specific peptideLane 2 : TNF-a treated 293 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-IKK gamma (phospho S85) antibody - ab63551 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IKK gamma (phospho S85) antibody (ab63551) at 1/500 dilutionLane 1 : extracts from HepG2 cells, treated with Anisomycin (0.5uM, 5hours)Lane 2 : extracts from HepG2 cells, treated with Anisomycin (0.5uM, 5hours) with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-IKK gamma (phospho S85) antibody - ab195921 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-IKK gamma (phospho S85) antibody (ab195921) at 1/500 dilution (antigen-specific peptide)Lane 2 : Anti-IKK gamma (phospho S85) antibody (ab195921) at 1/500 dilutionLane 1 : Anisomycin treated HepG2 whole cell lysates with antigen-specific peptideLane 2 : Anisomycin treated HepG2 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-IKK gamma antibody - ab137363 from Abcam

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-IKK gamma antibody (ab137363) at 1/3000 dilution + Jurkat cell lysate at 30 µg

Anti-IKK gamma antibody - ab155420 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-IKK gamma antibody (ab155420) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg

Anti-IKK gamma antibody - ab7890 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-IKK gamma antibody (ab7890) at 1 µg/ml + HeLa whole cell lysate

Anti-IKK gamma antibody - ab63255 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IKK gamma antibody (ab63255) at 1/500 dilutionLane 1 : extracts from HepG2 cells, treated with Anisomycin (0.5uM, 5hours), without immunising peptideLane 2 : extracts from HepG2 cells, treated with Anisomycin (0.5uM, 5hours), with immunising peptide

Anti-IKK gamma antibody - ab63538 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IKK gamma antibody (ab63538) at 1/500 dilutionLane 1 : Extracts from 293 cells (5ug)Lane 2 : Extracts from 293 cells (5ug), with immunizing non-phosphopeptide at 5 µg

Anti-IKK gamma antibody - ab14304 from Abcam

Prices $376.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
Anti-IKK gamma antibody (ab14304) at 1/2000 dilutionSecondaryGoat anti-IgY-HRP at 1/1000 dilution

Anti-IKK gamma antibody - ab69588 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IKK gamma antibody (ab69588) at 1/500 dilutionLane 1 : Cos7 cell extractLane 2 : Cos7 cell extract with peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-IKK gamma antibody - ab93392 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-IKK gamma antibody (ab93392) at 1/500 dilution + Interleukin 1 stimulated 293 cells

Anti-IKK gamma antibody - ab56358 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
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