Immunofluorescence analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling IKK gamma (green) with ab178872 at 1/250 dilution showing cytoplasm and nucleus staining. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution. Counter stain is labeling tubulin (red) with ab7291 at 1/500 dilution with secondary antibody Goat anti-Mouse AlexaFluor® 594 (ab150120) at 1/400 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab178872 at 1/250 dilution, ab150120 at 1/400 dilution. -ve control 2 is ab7291 at 1/500 dilution, ab150077 at 1/200 dilution. 

All lanes : Anti-IKK gamma [EPR16629] antibody (ab178872) at 1/20000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysatesLane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-IKK gamma [EPR16629] antibody (ab178872) at 1/5000 dilutionLane 1 : Human fetal brainLane 2 : Human fetal kidneyLane 3 : Human colon cancerLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-IKK gamma [EPR16629] antibody (ab178872) at 1/20000 dilutionLane 1 : Mouse brainLane 2 : Mouse heartLane 3 : Mouse kidneyLane 4 : Mouse spleenLane 5 : Rat brainLane 6 : Rat heartLane 7 : Rat kidneyLane 8 : Rat spleenLane 9 : C6 (Rat glial tumor cells) whole cell lysatesLane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysatesLane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysatesLane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin embedded human colonic adenocarcinoma tissue labeling IKK gamma with ab178872 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on colonic adenocarcinoma is observed.Negative control: Using PBS instead of primary ab, secondary ab ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit/Mouse IgG.

Immunohistochemical analysis of paraffin embedded Rat colon tissue labeling IKK gamma with ab178872 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stain is Hematoxylin. Cytoplasm staining on epithelial cells of rat colon is observed.Negative control: Using PBS instead of primary ab, secondary ab as above.

IKK gamma was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab178872 at 1/50 dilution. Western blot was performed of the immunoprecipitate using ab178872 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: HeLa whole cell extract. Right lane:  PBS instead of HeLa whole cell extract.Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM/TBST.