NDC80

Gene Symbol NDC80
Entrez Gene 10403
Alt Symbol HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80
Species Human
Gene Type protein-coding
Description NDC80 kinetochore complex component
Other Description NDC80 homolog, kinetochore complex component|NDC80 kinetochore complex component homolog|highly expressed in cancer protein|highly expressed in cancer, rich in leucine heptad repeats|kinetochore associated 2|kinetochore protein Hec1|kinetochore protein NDC80 homolog|kinetochore-associated protein 2|retinoblastoma-associated protein HEC
Swissprots Q6PJX2 O14777
Accessions CAM84948 CCQ77692 CCQ77701 CCQ77710 CCQ77719 CDH48198 CDH61620 CDH61674 CDH61693 CDH88991 CDH92529 CDH92543 CDH92554 CDH92801 CDH92898 CDI44673 CDI44682 EAX01696 EAX01697 O14777 AF017790 AAB80726 AK289396 BAF82085 AK313184 BAG36001 BC005239 AAH05239 BC010171 BC035617 AAH35617 DQ890765 ABM81691 DQ893928 ABM84854 NM_006101 NP_006092
Function Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. {ECO:0000269|PubMed:12351790, ECO:0000269|PubMed:14654001, ECO:0000269|PubMed:14699129, ECO:0000269|PubMed:15062103, ECO:0000269|PubMed:15235793, ECO:0000269|PubMed:15239953, ECO:0000269|PubMed:15548592, ECO:0000269|PubMed:16732327, ECO:0000269|PubMed:9315664}.
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.

Hec1 (N-16) - sc-26106 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Hec1 (T-19) - sc-26107 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Hec1 (1) - sc-135934 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
Hec1 (1): sc-135934. Western blot analysis of Hec1 expression in Jurkat whole cell lysate.

Anti-HEC1 antibody - ab186839 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HEC1 antibody (ab186839) at 1/500 dilutionLane 1 : K562 cell lysateLane 2 : LOVO cell lysateLane 3 : MCF7 cell lysateLane 4 : 22RV-1 cell lysateLane 5 : 293T cell lysateLane 6 : mouse testis lysateLane 7 : THP1 cell lysate

Anti-HEC1 antibody - ab97610 from Abcam

Prices $376.00
Sizes 100 µl
Host Goat
Clonality Polyclonal
All lanes : Anti-HEC1 antibody (ab97610) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µg

Anti-HEC1 antibody - ab117415 from Abcam

Prices $384.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab117415, at 5 ug/ml staining HEC1 in Formalin-fixed, Paraffin-embedded Human adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-HEC1 antibody - ab69978 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HEC1 antibody (ab69978) at 1/500 dilutionLane 1 : Jurkat cell extractsLane 2 : Jurkat cell extracts with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-HEC1 antibody - ab35651 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : MarkerLane 2 : Anti-HEC1 antibody (ab35651) at 1 µg/mlLane 1 : As aboveLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryLane 2 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-HEC1 antibody - ab172396 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-HEC1 antibody (ab172396) at 1 µg/mlLane 1 : HEC1 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-HEC1 antibody - ab3393 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-HEC1 antibody - ab70824 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HEC1 antibody [9G3] - ab3613 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 9G3
Anti-Hec1 antibody (ab3613) labels the kinetochores of mitotic cells in LLCPK1 (Sus scrofa kidney epithelial cell line) cell lines. Merge shows an overlay of DNA (stained with DAPI, red) and Hec1 (green).This image was kindly supplied as part of the review submitted by Marko Kallio.

Anti-HEC1 antibody [EPR5342] - ab109496 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5342
All lanes : Anti-HEC1 antibody [EPR5342] (ab109496) at 1/10000 dilutionLane 1 : Jurkat lysateLane 2 : HeLa lysateLane 3 : NCCIT lysateLane 4 : Human spleen lysateLysates/proteins at 10 µg per lane.

Anti-HEC1 / NDC80 Antibody - LS-C48686 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HEC1 / NDC80 Antibody - LS-C73202 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-HEC1 / NDC80 Antibody (Internal) - LS-C145119 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HEC1 / NDC80 Antibody (aa600-642) - LS-C190203 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HEC1 / NDC80 Antibody (C-Terminus) - LS-C203197 from LifeSpan Bioscience

Prices $245.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal

Anti-HEC1 / NDC80 Antibody (C-Terminus) - LS-C287627 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HEC1 / NDC80 Antibody (aa545-642, Azide-free) - LS-C330554 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-HEC1 / NDC80 Antibody - LS-C109442 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample. A: 30g of A431 whole cell lysate. B: 30g of H1299 whole cell lysate. 7.5% SDS PAGE. NDC80 antibody diluted at 1:1000

Anti-HEC1 / NDC80 Antibody - LS-C341217 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HEC1 / NDC80 Antibody (clone 2497C3a) - LS-C184492 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2497C3a
Western blot of immunized recombinant protein using KNTC2 antibody.

Anti-HEC1 / NDC80 Antibody (aa351-400) - LS-C118661 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HUVEC cells, using KNTC2 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-HEC1 / NDC80 Antibody (C-Terminus, clone EPR5342) - LS-C138396 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5342
Western blot analysis on (A) Jurkat, (B) HeLa, (C) NCCIT, and (D) human spleen lysates using anti-Ndc80 antibody.
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