ID4

Gene Symbol	ID4
Entrez Gene	3400
Alt Symbol	IDB4, bHLHb27
Species	Human
Gene Type	protein-coding
Description	inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
Other Description	DNA-binding protein inhibitor ID-4\|class B basic helix-loop-helix protein 27\|inhibitor of differentiation 4
Swissprots	Q13005 P47928
Accessions	AAC39674 EAW55416 P47928 AI692586 AJ420553 AK130851 BC014941 AAH14941 DB461864 U16153 AAA82882 U28368 AAA73923 Y07958 CAA69255 NM_001546 NP_001537
Function	Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation (By similarity). {ECO:0000250}.
Subcellular Location	Nucleus.
Top Pathways	TGF-beta signaling pathway, Signaling pathways regulating pluripotency of stem cells

Id4 (B-5) - sc-365656 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Id4 (B-5): sc-365656. Western blot analysis of Id4 expression in non-transfected: sc-117752 (A) and human Id4 transfected: sc-110122 (B) 293T whole cell lysates.

Id4 (H-70) - sc-13047 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Id4 (H-70): sc-13047. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear and cytoplasmic localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

Id4 (C-17) - sc-27190 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Id4 (C-17): sc-27190. Western blot analysis of Id4 expression in non-transfected: sc-117750 (A) and human Id4 transfected: sc-110122 (B) whole cell lysates.

Id4 (L-20) - sc-491 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Id4 (L-20): sc-491. Western blot analysis of Id4 expression in non-transfected: sc-117752 (A) and human Id4 transfected: sc-110122 (B) 293T whole cell lysates.

Id4 (N-13) - sc-27188 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-ID4 antibody - ab49261 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 antibody (ab49261) at 1.25 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG 1/50000-1/100000

Anti-ID4 antibody - ab168414 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-ID4 antibody (ab168414) at 1 µg/mlLane 1 : ID4 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysate

Anti-ID4 antibody - ab86113 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 antibody (ab86113) at 1 µg/ml (5% skim milk/PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 10-20%SecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-ID4 antibody - ab136819 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 antibody (ab136819) at 1/500 dilution + HeLa cell lysate at 30 µg

Anti-ID4 antibody - N-terminal - ab189551 from Abcam

Host	Rabbit
Clonality	Polyclonal

Anti-ID4 antibody - N-terminal (ab189551) at 1/1000 dilution + Mouse thymus total cell extract

Anti-ID4 Antibody (clone 13A200) - LS-C183391 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	13A200

Anti-ID4 Antibody (N-Terminus) - LS-C30577 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody (aa71-120) - LS-C101385 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ID4 antibody LS-C101385 Western blot of 293T cell lysate.

Anti-ID4 Antibody (Ser5) - LS-C177168 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Id4 (S5) pAb in extracts from HepG2 cells.

Anti-ID4 Antibody (aa1-30, AP) - LS-C222841 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody (aa1-30, APC) - LS-C222843 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody (aa1-30, Biotin) - LS-C222844 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody (aa1-30, FITC) - LS-C222845 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody (aa1-30, Azide-free, HRP) - LS-C226802 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody (aa1-30, PE) - LS-C226803 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody (aa5-54) - LS-C292083 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody - LS-C308372 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-ID4 Antibody (aa1-45) - LS-C311577 from LifeSpan Bioscience

Prices	$250.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody (aa1-30) - LS-C322145 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ID4 Antibody (clone 3H1) - LS-C339426 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3H1

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID4.

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