Gene Symbol | Otub1 |
---|---|
Entrez Gene | 107260 |
Alt Symbol | AI850305 |
Species | Mouse |
Gene Type | protein-coding |
Description | OTU domain, ubiquitin aldehyde binding 1 |
Other Description | OTU domain-containing ubiquitin aldehyde-binding protein 1|deubiquitinating enzyme OTUB1|otubain 1|otubain-1|ubiquitin thioesterase OTUB1|ubiquitin-specific-processing protease OTUB1 |
Swissprots | Q3ULV9 Q7TQI3 Q8C326 Q8R5F2 Q3V408 |
Accessions | EDL33291 Q7TQI3 AK027996 BAE20433 AK087195 AK140070 BAE24227 AK145273 BAE26339 AK145970 BAE26795 AK191684 AK217174 BC022575 AAH22575 BC054410 AAH54410 NM_134150 NP_598911 |
Function | Hydrolase that can specifically remove compared to 'Lys- 48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'- linked ubiquitin. {ECO:0000269|PubMed:14661020}. |
Subcellular Location | Cytoplasm {ECO:0000250}. |
OTUB1 CRISPR/Cas9 KO Plasmid (m) - sc-430702 from Santa Cruz Biotechnology
|
||||||||
OTUB1 HDR Plasmid (m) - sc-430702-HDR from Santa Cruz Biotechnology
|
||||||||
OTUB1 Double Nickase Plasmid (m) - sc-430702-NIC from Santa Cruz Biotechnology
|
||||||||
OTUB1 Double Nickase Plasmid (m2) - sc-430702-NIC-2 from Santa Cruz Biotechnology
|
||||||||
OTUB1 CRISPR Activation Plasmid (m) - sc-430702-ACT from Santa Cruz Biotechnology
|
||||||||
OTUB1 CRISPR Activation Plasmid (m2) - sc-430702-ACT-2 from Santa Cruz Biotechnology
|
||||||||
OTUB1 Lentiviral Activation Particles (m) - sc-430702-LAC from Santa Cruz Biotechnology
|
||||||||
OTUB1 Lentiviral Activation Particles (m2) - sc-430702-LAC-2 from Santa Cruz Biotechnology
|
||||||||
OTUB1 siRNA (m) - sc-76015 from Santa Cruz Biotechnology
|
||||||||
OTUB1 shRNA Plasmid (m) - sc-76015-SH from Santa Cruz Biotechnology
|
||||||||
OTUB1 shRNA (m) Lentiviral Particles - sc-76015-V from Santa Cruz Biotechnology
|
||||||||
MISSION® esiRNA - EMU016031 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN38127 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND38127 from Sigma-Aldrich
|
||||||||
OTUB1 siRNA (Mouse) - CRN0817 from Cohesion Biosciences
|
||||||||
shRNA set against Mouse OTUB1 (NM_134150.2) - SHH369080 from Creative biogene
|
||||||||
shRNA set against Mouse Otub1(NM_134150.2) - SHR083472 from Creative biogene
|
||||||||
Mouse OTUB1 siRNA - orb290292 from Biorbyt
|
||||||||
OTUB1 shRNA Plasmid (Mouse) - CHN0817 from Cohesion Biosciences
|
||||||||
OTUB1 miRNA 3'UTR clone - MiUTR1M-08812 from Creative biogene
|