Tnf

Gene Symbol	Tnf
Entrez Gene	21926
Alt Symbol	DIF, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnfa, Tnfsf1a
Species	Mouse
Gene Type	protein-coding
Description	tumor necrosis factor
Other Description	cachectin\|tumor necrosis factor ligand superfamily member 2\|tumor necrosis factor-alpha
Swissprots	Q91VF3 Q62326 O35853 P06804
Accessions	AAA18594 AAA40459 AAA40462 AAB65593 AAC82484 BAA19512 BAA19513 BAB68748 BAB68750 BAB68751 BAB68752 BAB68753 BAB68754 BAB68755 BAB68756 BAB78397 BAF02298 BAF02299 BAF02300 CAA68530 EDL26631 EDL26632 P06804 AK153319 BAE31899 AK153800 BAE32187 AK154223 BAE32445 AK155964 BAE33525 AY423855 AAR00346 BC117057 AAI17058 BC137720 AAI37721 BY167004 M11731 AAA40458 M13049 AAA40457 X02611 CAA26457 NM_001278601 NP_001265530 NM_013693 NP_038721
Function	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Subcellular Location	C-domain 2: Secreted {ECO:0000250}.
Top Pathways	T cell receptor signaling pathway, Allograft rejection, Systemic lupus erythematosus, HTLV-I infection, Influenza A

TNF-α (D2D4) XP ® Rabbit mAb (Mouse Specific) (PE Conjugate) - 12779 from Cell Signaling Technology

Prices	$329.00
Sizes	100 µl (50 tests)
Host	Rabbit
Clonality	Polyclonal

Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment) (green), using TNF-α (D2D4) XP ® Rabbit mAb (Mouse Specific) (PE Conjugate).

TNF-α (D2D4) XP ® Rabbit mAb (Mouse Specific) - 11948 from Cell Signaling Technology

Prices	$109.00, $277.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D2D4

Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated (+) with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using TNF-α (D2D4) XP ® Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Mouse TNF-α Neutralizing (D2H4) Rabbit mAb - 11969 from Cell Signaling Technology

Prices	$338.00
Sizes	100 µg
Host	Rabbit
Clonality	Monoclonal
Clone	D2H4

The ability of Mouse TNF-α Neutralizing (D2H4) Rabbit mAb to inhibit mTNF-α-induced L-929 cell cytotoxicity was assessed. Cells were incubated with increasing concentrations of antibody in the presence of mTNF-α #5178 (250 pg/ml) and 1 µg/ml actinomycin D. After 24 hr, viable cells were detected by incubation with a tetrazolium salt and the OD 450 was determined.

TNFα (R-19) - sc-1349 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Western blot analysis of mouse recombinant TNFα. Antibodies tested include TNFα (M-18): sc-1348 (A,B) and TNFα (R-19): sc-1349 (C,D).

TNFα (TN3-19.12) - sc-12744 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Hamster
Clonality	Monoclonal

TNFα (TN3-19.12): sc-12744. Western blot analysis of mouse recombinant TNFα.

Anti-TNF alpha antibody - ab34674 from Abcam

Prices	$394.00
Sizes	500 µg
Host	Rabbit
Clonality	Polyclonal

ab34674 staining TNF alpha in mouse knee joint sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 10 minutes at room temperature followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes. An undiluted HRP-conjugated human anti-rabbit monoclonal was used as secondary antibody.See Abreview

Anti-TNF alpha antibody (Biotin) - ab17611 from Abcam

Host	Goat
Clonality	Polyclonal

Anti-TNF alpha antibody [MP6-XT22] - ab34719 from Abcam

Prices	$373.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	MP6-XT22

Anti-TNF alpha antibody [MP6-XT3] - ab11564 from Abcam

Prices	$379.00
Sizes	125 µg
Host	Rat
Clonality	Monoclonal
Clone	MP6-XT3

Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha (ab11564) as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detetction antibody and Streptavidin:HRP as the final layer.

Anti-TNF alpha antibody [MP6-XT3] (Biotin) - ab24930 from Abcam

Prices	$372.00
Sizes	250 µg
Host	Rat
Clonality	Monoclonal
Clone	MP6-XT3

Anti-TNF alpha antibody [TN3-19] - Low endotoxin, Azide free - ab185795 from Abcam

Prices	$368.00
Sizes	50 µg
Clonality	Monoclonal
Clone	TN3-19

Anti-TNF alpha antibody [TN3-19.12] - ab10863 from Abcam

Prices	$378.00
Sizes	50 µl
Clonality	Monoclonal
Clone	TN3-19.12

Anti-TNF alpha antibody [V1q(H8)] - ab8097 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	V1q(H8)

TNF-alpha Antibody - AF-410-NA from Novus Biologicals

Prices	$99.00, $369.00
Sizes	25 µg, 100 µg
Host	Goat
Clonality	Polyclonal

Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND50 is typically 0.1-0.4 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNF-alpha Antibody (MP6-XT22) - MAB4101 from Novus Biologicals

Prices	$99.00, $259.00
Sizes	25 µg, 100 µg
Host	Rat
Clonality	Monoclonal
Clone	MP6-XT22

TNF-alpha Antibody (TN3-19.12) [Unconjugated] - MAB410 from Novus Biologicals

Prices	$99.00, $269.00, $699.00
Sizes	25 µg, 100 µg, 500 µg
Host	Hamster
Clonality	Monoclonal
Clone	TN3-19.12

TNF-alpha Antibody - AB-410-NA from Novus Biologicals

Prices	$359.00
Sizes	1 mg
Host	Goat
Clonality	Polyclonal

TNF-alpha Antibody (TN3-19.12) - NB110-9916 from Novus Biologicals

Prices	$349.00
Sizes	100 µl
Host	Hamster
Clonality	Monoclonal
Clone	TN3-19.12

TNF-alpha Antibody (MP6-XT3) - NBP2-25180 from Novus Biologicals

Prices	$589.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	MP6-XT3

ELISA: TNF-alpha Antibody (MP6-XT3) [NBP2-25180] - Recombinant Mouse TNF alpha (PMP24) detected in a sandwich assay with Rat anti Mouse TNF alpha as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detection antibody and Streptavidin:HRP as the final layer.

TNF-alpha Antibody (V1q) - NBP1-51500 from Novus Biologicals

Prices	$329.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	V1q

TNF-alpha Antibody (MP6-XT3) - NB600-781 from Novus Biologicals

Prices	$349.00
Sizes	25 µg
Host	Rat
Clonality	Monoclonal
Clone	MP6-XT3

ELISA: TNF alpha Antibody (MP6-XT3) [NB600-781] Recombinant Mouse TNF alpha (PMP24) detected in a sandwich assay with Rat anti Mouse TNF alpha as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detection antibody and Streptavidin:HRP as the final layer

TNF-alpha Antibody - NBP1-77867 from Novus Biologicals

Prices	$429.00
Sizes	1 ml
Host	Rabbit
Clonality	Polyclonal

TNF-alpha Antibody (MP6-XT22) - NBP2-27518 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	MP6-XT22

TNF-alpha Antibody (6B8) - NB100-78234 from Novus Biologicals

Prices	$349.00
Sizes	500 µg
Host	Rat
Clonality	Monoclonal
Clone	6B8

Sandwich ELISA: TNF alpha Antibody (6B8) [NB100-78234] - The purified 6B8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP6-XT22 antibody. A concentration range of 2-6 ug/ml is recommended.

TNF-alpha Antibody [Biotin] - BAF410 from Novus Biologicals

Prices	$420.00
Sizes	50 µg
Host	Goat
Clonality	Polyclonal

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