Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND50 is typically 0.1-0.4 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
TNF‑ alpha was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TNF‑ alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑410‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.