Anti-TNF alpha antibody
| Name | Anti-TNF alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab34674 |
| Prices | $394.00 |
| Sizes | 500 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP WB IHC-P IHC-F ELISA RIA B/N |
| Species Reactivities | Mouse |
| Antigen | Recombinant TNF alpha from mouse, produced in E |
| Blocking Peptide | TNF alpha peptide |
| Description | Rabbit Polyclonal |
| Gene | Tnf |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab34674 staining TNF alpha in mouse knee joint sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 10 minutes at room temperature followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes. An undiluted HRP-conjugated human anti-rabbit monoclonal was used as secondary antibody.See Abreview
ab34674 staining TNF alpha in immortalised bone marrow-derived macrophages by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA + 5% goat serum in PBS for 1 hour at 21°C. The primary antibody was diluted 1/200 in the blocking buffer and incubated with the sample for 1 hour at 21°C. The secondary antibody was Alexa Fluor® 568-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.See Abreview
Product References
Wnt4 signaling prevents skeletal aging and inflammation by inhibiting nuclear - Wnt4 signaling prevents skeletal aging and inflammation by inhibiting nuclear
Yu B, Chang J, Liu Y, Li J, Kevork K, Al-Hezaimi K, Graves DT, Park NH, Wang CY. Nat Med. 2014 Sep;20(9):1009-17.
Breakdown of immune homeostasis in the testis of mice lacking Tyro3, Axl and Mer - Breakdown of immune homeostasis in the testis of mice lacking Tyro3, Axl and Mer
Zhang Y, Li N, Chen Q, Yan K, Liu Z, Zhang X, Liu P, Chen Y, Han D. Immunol Cell Biol. 2013 Jul;91(6):416-26.
TNFalpha reverse signaling promotes sympathetic axon growth and target - TNFalpha reverse signaling promotes sympathetic axon growth and target
Kisiswa L, Osorio C, Erice C, Vizard T, Wyatt S, Davies AM. Nat Neurosci. 2013 Jul;16(7):865-73.
Therapeutic potential of a novel cannabinoid agent CB52 in the mouse model of - Therapeutic potential of a novel cannabinoid agent CB52 in the mouse model of
Ribeiro R, Yu F, Wen J, Vana A, Zhang Y. Neuroscience. 2013 Dec 19;254:427-42.
Disturbed follicular architecture in B cell A disintegrin and metalloproteinase - Disturbed follicular architecture in B cell A disintegrin and metalloproteinase
Folgosa L, Zellner HB, El Shikh ME, Conrad DH. J Immunol. 2013 Dec 15;191(12):5951-8.
Neutralization of granulocyte macrophage colony-stimulating factor decreases - Neutralization of granulocyte macrophage colony-stimulating factor decreases
Manczak M, Mao P, Nakamura K, Bebbington C, Park B, Reddy PH. Hum Mol Genet. 2009 Oct 15;18(20):3876-93.