Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑ alpha Polyclonal Antibody (Catalog # AB-410-NA). The ND50 is typically 0.2-0.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).