Gene Symbol | EME1 |
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Entrez Gene | 146956 |
Alt Symbol | MMS4L, SLX2A |
Species | Human |
Gene Type | protein-coding |
Description | essential meiotic structure-specific endonuclease 1 |
Other Description | MMS4 homolog|SLX2 structure-specific endonuclease subunit homolog A|crossover junction endonuclease EME1|essential meiotic endonuclease 1 homolog 1|essential meiotic endonuclease 1 homolog 2|hMMS4|homolog of yeast EME1 endonuclease |
Swissprots | Q96N62 Q96AY2 |
Accessions | EAW94617 EAW94618 EAW94619 EAW94620 EAW94621 Q96AY2 AK021607 AK055926 BAB71047 AK308275 BC016470 AAH16470 DA006645 DB245967 DQ578819 JF432239 ADZ15456 XM_005257081 XP_005257138 XM_011524392 XP_011522694 XM_011524393 XP_011522695 XM_011524394 XP_011522696 XM_011524395 XP_011522697 XM_011524396 XP_011522698 XR_934390 XR_934392 XR_934393 NM_001166131 NP_001159603 NM_152463 NP_689676 |
Function | Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'- flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. {ECO:0000269|PubMed:12686547, ECO:0000269|PubMed:12721304, ECO:0000269|PubMed:14617801, ECO:0000269|PubMed:17289582}. |
Subcellular Location | Nucleus, nucleolus {ECO:0000269|PubMed:14617801}. Note=Recruited to regions of DNA damage in S-phase cells. |
Top Pathways | Homologous recombination, Fanconi anemia pathway |
Eme1 CRISPR/Cas9 KO Plasmid (h) - sc-403404 from Santa Cruz Biotechnology
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Eme1 HDR Plasmid (h) - sc-403404-HDR from Santa Cruz Biotechnology
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Eme1 Double Nickase Plasmid (h) - sc-403404-NIC from Santa Cruz Biotechnology
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Eme1 Double Nickase Plasmid (h2) - sc-403404-NIC-2 from Santa Cruz Biotechnology
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Eme1 CRISPR Activation Plasmid (h) - sc-403404-ACT from Santa Cruz Biotechnology
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Eme1 CRISPR Activation Plasmid (h2) - sc-403404-ACT-2 from Santa Cruz Biotechnology
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Eme1 Lentiviral Activation Particles (h) - sc-403404-LAC from Santa Cruz Biotechnology
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Eme1 Lentiviral Activation Particles (h2) - sc-403404-LAC-2 from Santa Cruz Biotechnology
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Eme1 siRNA (h) - sc-72080 from Santa Cruz Biotechnology
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Eme1 shRNA Plasmid (h) - sc-72080-SH from Santa Cruz Biotechnology
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Eme1 shRNA (h) Lentiviral Particles - sc-72080-V from Santa Cruz Biotechnology
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EME1 RNAi - H00146956-R02 from Novus Biologicals
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CompoZr® Knockout ZFN Kit - CKOZFN1392 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1391 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1392 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1391 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR04209 from Sigma-Aldrich
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EME1 siRNA (Human) - CRJ5504 from Cohesion Biosciences
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EME1 - MBS8214669 from MyBioSource
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shRNA set against Human EME1 (NM_152463.2) - SHH285413 from Creative biogene
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Human EME1 siRNA - orb276005 from Biorbyt
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EME1 shRNA Plasmid (Human) - CHJ5504 from Cohesion Biosciences
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EME1 miRNA 3'UTR clone - MiUTR3H-11531 from Creative biogene
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EME1 miRNA 3'UTR clone - MiUTR3H-11532 from Creative biogene
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