PPP1R12A

Gene Symbol PPP1R12A
Entrez Gene 4659
Alt Symbol M130, MBS, MYPT1
Species Human
Gene Type protein-coding
Description protein phosphatase 1, regulatory subunit 12A
Other Description myosin binding subunit|myosin phosphatase, target subunit 1|myosin phosphatase-targeting subunit 1|protein phosphatase 1 regulatory subunit 12A|protein phosphatase 1, regulatory (inhibitor) subunit 12A|protein phosphatase myosin-binding subunit
Swissprots Q86WU3 Q9BYH0 B4DZ09 Q2NKL4 O14974 Q8NFR6 F8VWB4 Q569H0
Accessions BAB39107 EAW97351 EAW97352 EAW97353 EAW97354 EAW97355 EAW97356 O14974 AF086032 AF458589 AAM49717 AK057712 AK125671 AK302692 BAG63921 AK314193 BAG36872 AY380574 AAQ88438 BC000812 BC013941 AAH13941 BC040375 BC047898 AAH47898 BC062560 BC092481 AAH92481 BC111752 AAI11753 D87930 BAA22378 DC350703 XM_005268887 XP_005268944 XM_011538374 XP_011536676 XM_011538375 XP_011536677 XM_011538376 XP_011536678 XM_011538377 XP_011536679 XM_011538378 XP_011536680 XM_011538379 XP_011536681 XM_011538380 XP_011536682 XM_011538381 XP_011536683 XM_011538382 XP_011536684 XM_011538383 XP_011536685 XM_011538384 XP_011536686 XM_011538385 XP_011536687 XR_944554 NM_001143885 NP_001137357 NM_001143886 NP_001137358 NM_001244990 NP_001231919 NM_001244992 NP_001231921 NM_002480 NP_002471
Function Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity. {ECO:0000269|PubMed:18477460, ECO:0000269|PubMed:19245366, ECO:0000269|PubMed:20354225}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:12923170, ECO:0000269|PubMed:18477460}. Note=Along actomyosin filaments and stress fibers.
Tissue Specificity Expressed in striated muscles, specifically in type 2a fibers (at protein level). {ECO:0000269|PubMed:20634291}.
Top Pathways Focal adhesion, Vascular smooth muscle contraction, Oxytocin signaling pathway, cGMP-PKG signaling pathway, cAMP signaling pathway

Phospho-MYPT1 (Ser507) Antibody - 3040 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with calyculin A #9902, using Phospho-MYPT1 (Ser507) Antibody (upper) or MYPT1 Antibody #2634 (lower).

Phospho-MYPT1 (Ser668) Antibody - 3048 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with Calyculin A #9902, using Phospho-MYPT1 (Ser668) Antibody (upper) or total MYPT1 Antibody #2634 (lower).

Phospho-MYPT1 (Thr696) Antibody - 5163 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated or treated with the ROCK inhibitor Y27632 (2 minutes at 10 μM) using Phospho-MYPT1 (Thr696) Antibody or total MYPT1 Antibody #2634. Phospho-MYPT1 (Thr696) Antibody was pre-incubated as indicated with phospho-MYPT1 (Thr696) peptide or nonphospho-MYPT1 (Thr696) peptide.

Phospho-MYPT1 (Thr853) Antibody - 4563 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Phospho-MYPT1 (Thr853) Antibody.

MYPT1 Antibody - 2634 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using MYPT1 Antibody.

MYPT1 (D6C1) Rabbit mAb - 8574 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6C1
Western blot analysis of extracts from various cell lines using MYPT1 (D6C1) Rabbit mAb.

p-MYPT1 (A-6) - sc-377542 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of MYPT1 phosphorylation in untreated (A,D), induction cocktail (sc-362324) treated (B,E) and induction cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MYPT1 (A-6): sc-377542 (A,B,C) and MYPT1 (H-130): sc-25618 (D,E,F).

MYPT1 (C-6) - sc-514261 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MYPT1 (C-6): sc-514261. Western blot analysis of MYPT1 expression in DU 145 (A), HeLa (B) and Jurkat (D) whole cell lysates and HeLa nuclear extract (C).

MYPT1 (E-19) - sc-17434 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MYPT1 (E-19): sc-17434. Western blot analysis of MYPT1 expression in 3T3-L1 whole cell lysate.

MYPT1 (H-130) - sc-25618 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MYPT1 (H-130): sc-25618. Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tumor showing cytoplasmic localization in selected cells.

MYPT1 (K-18) - sc-34142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MYPT1 (K-18): sc-34142. Western blot analysis of MYPT1 expression in HeLa (A), U-251-MG (B) and HEK293 (C) whole cell lysates and HeLa nuclear extract (D).

MYPT1 (N-15) - sc-17433 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MYPT1 (N-15): sc-17433. Western blot analysis of MYPT1 expression in non-transfected 293T: sc-117752 (A), human MYPT1 transfected 293T: sc-114575 (B) and 3T3-L1 (C) whole cell lysates.

MYPT1/2 (C-18) - sc-34143 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MYPT1/2 (C-18): sc-34143. Western blot analysis of MYPT1/2 expression in HeLa nuclear extract.

p-MYPT1 (F-11) - sc-377531 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of MYPT1 phosphorylation in untreated (A,D), Calyculin A treated (B,E) and Calyculin A and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MYPT1 (F-11): sc-377531 (A,B,C) and MYPT1 (H-130): sc-25618 (D,E,F).

p-MYPT1 (Ser 695) - sc-33360 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-MYPT1 (Ser 695): sc-33360. Western blot analysis of phosphorylated MYPT1 expression in non-transfected: sc-110760 (A) and human MYPT1 transfected: sc-111087 (B) 293 whole cell lysates.

p-MYPT1 (Ser 903) - sc-17557 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of MYPT1 phosphorylation in untreated (A, D), calyculin treated (B, E) and calyculin and lambda protein phosphatase treated (C, F) HeLa whole cell lysates. Antibodies tested include p-MYPT1 (Ser 903): sc-17557 (A, B, C) and MYPT1 (H-130): sc-25618 (D, E, F).

p-MYPT1 (Thr 696) - sc-17556 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-MYPT1 (Thr 853) - sc-17432 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Myosin Phosphatase (phospho S903) antibody [EPR2373(2)] - ab181181 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2373(2)
All lanes : Anti-Myosin Phosphatase (phospho S903) antibody [EPR2373(2)] (ab181181) at 1/50000 dilutionLane 1 : Okadaic acid and Calyculin A treated HEK293 cell lysateLane 2 : Untreated HEK293 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Myosin Phosphatase (phospho T696) antibody - ab59202 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59202 at 1/50 dilution. Samples were untreated (left) or treated with immunizing peptide (right).

Anti-Myosin Phosphatase (phospho T853) antibody - ab59203 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Myosin Phosphatase (phospho T853) antibody (ab59203) at 1/500 dilutionLane 1 : NIH/3T3 cell extractLane 2 : NIH/3T3 cell extract with immunizing phosphopeptide

Anti-Myosin Phosphatase 1+Myosin Phosphatase 2 antibody [YE336] - ab32519 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone YE336
Anti-Myosin Phosphatase 1+Myosin Phosphatase 2 antibody [YE336] (ab32519) at 1/5000 dilution + 293T cell lysate.

Anti-Myosin Phosphatase antibody - ab59235 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Myosin Phosphatase antibody (ab59235) at 1/500 dilutionLane 1 : NIH3T3 cell extractLane 2 : NIH3T3 cell extract with immunising peptide

Anti-Myosin Phosphatase antibody - ab24670 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-Myosin Phosphatase antibody - ab131094 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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