ab32519 at a 1:100 dilution staining Myosin Phosphatase 1 / Myosin Phosphatase 2 in 293 cells using Immunocytochemistry/Immunofluorescence.

ab32519 staining mouse fibroblast cells by ICC/IF. Cells were PFA fixed, permeabilized in Triton X-100 and blocked in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with sample for 4 hours at 25°C. An Alexa Fluor® 488 conjugated goat monoclonal to rabbit, diluted 1/500 was used as the secondary.See Abreview

Overlay histogram showing HEK293 cells stained with ab32519 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32519, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.