RAD9A

Gene Symbol RAD9A
Entrez Gene 5883
Alt Symbol RAD9
Species Human
Gene Type protein-coding
Description RAD9 homolog A (S. pombe)
Other Description DNA repair exonuclease rad9 homolog A|cell cycle checkpoint control protein RAD9A|hRAD9
Swissprots B2RCZ8 Q96C41 Q6FI29 Q99638
Accessions AAU89725 BAH69085 BAH69086 BAH69087 EAW74604 EAW74605 EAW74606 Q99638 AA708970 AK310213 AK315348 BAG37745 BC014848 AAH14848 CB164642 CN402466 CR536508 CAG38746 U53174 AAB39928 XM_006718652 XP_006718715 XM_011545205 XP_011543507 XR_428922 NM_001243224 NP_001230153 NM_004584 NP_004575
Function Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Subcellular Location Nucleus {ECO:0000269|PubMed:12628935}.

Rad9A (D2J4P) Rabbit mAb - 14484 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2J4P
Western blot analysis of extracts from various cell lines using Rad9A (D2J4P) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Rad9 (A-4) - sc-74463 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rad9 (A-4): sc-74463. Western blot analysis of Rad9 expression in HeLa whole cell lysate (A) and HeLa (B) and KNRK (C) nuclear extracts.

Rad9 (B-6) - sc-166457 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rad9 (B-6): sc-166457. Western blot analysis of Rad9 expression in non-transfected: sc-117752 (A) and human Rad9 transfected: sc-113776 (B) 293T whole cell lysates.

Rad9 (C-20) - sc-10465 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rad9 (C-20): sc-10465. Western blot analysis of mouse recombinant Rad9 fusion protein.

Rad9 (B-8) - sc-74464 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rad9 (B-8): sc-74464. Western blot analysis of Rad9 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates and HeLa (C) and KNRK (D) neclear extracts.

Rad9 (M-389) - sc-8324 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rad9 (M-389): sc-8324. Western blot analysis of Rad9 expression in HeLa (A) and KNRK (B) whole cell lysates.

Rad9 (S-20) - sc-32489 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rad9 (S-20): sc-32489. Western blot analysis of Rad9 expression in non-transfected: sc-117752 (A) and human Rad9 transfected: sc-113776 (B) 293T whole cell lysates.

Rad9 (56) - sc-136053 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
Rad9 (56): sc-136053. Western blot analysis of Rad9 expression in human endothelial whole cell lysate.

p-Rad9 (Ser 277) - sc-130213 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Rad9 (Ser 277): sc-130213. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.

Anti-Rad9 antibody - ab96862 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad9 antibody (ab96862) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : HeLa whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Rad9 antibody - ab59022 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Rad9 antibody (ab59022) at 0.3 µg/ml + A431 cell lysate (35µg protein in RIPA buffer)

Anti-Rad9 antibody - ab63049 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Rad9 antibody (ab63049) at 1/60 dilution + Hela cell line lysates at 35 µg

Anti-Rad9 antibody - ab84912 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84912, at 1/250 dilution, staining Rad9 in formalin-fixed, paraffin-embedded human prostate carcinoma by Immunohistochemistry using DAB staining.

Anti-Rad9 antibody - ab50311 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Rad9 antibody [3A3-A7-F8] - ab150747 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A3-A7-F8
All lanes : Anti-Rad9 antibody [3A3-A7-F8] (ab150747) at 1/500 dilutionLane 1 : Rat testis cell lysatesLane 2 : MCF7 cell lysatesLane 3 : COS7 cell lysatesLane 4 : HeLa cell lysatesLane 5 : 3T3 cell lysates

Anti-Rad9 antibody [7B11] - ab117848 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7B11
All lanes : Anti-Rad9 antibody [7B11] (ab117848) at 1/2000 dilutionLane 1 : HepG2 lysateLane 2 : HeLa lysateLane 3 : HT29 lysateLane 4 : A549 lysateLane 5 : COS7 lysateLane 6 : Jurkat lysateLane 7 : MDCK lysateLane 8 : PC12 lysateLane 9 : MCF7 lysateLysates/proteins at 35 µg per lane.

Anti-Rad9 antibody [93A535] - ab13600 from Abcam

Prices $391.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 93A535
IHC image of Rad9 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13600, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Rad9 antibody - ChIP Grade - ab70810 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Rad9 with ab70810 at 1/1000 (1µg/ml). Detection: DAB.

Anti-RAD9A / RAD9 Antibody - LS-C17551 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-RAD9A / RAD9 Antibody (aa1125-1139) - LS-C42615 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RAD9A / RAD9 Antibody - LS-C93554 from LifeSpan Bioscience

Prices $435.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-RAD9A / RAD9 Antibody (aa13-33) - LS-C93557 from LifeSpan Bioscience

Prices $650.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-RAD9A / RAD9 Antibody (aa377-390) - LS-C55357 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.3 ug/ml) staining of A431cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-RAD9A / RAD9 Antibody (C-Terminus) - LS-C82028 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-RAD9A / RAD9 Antibody (clone 93A535) - LS-C543 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 93A535
Western blot of RAD9 using antibody at 2 ug/ml against recombinant RAD9.
Search more