ALYREF

Gene Symbol ALYREF
Entrez Gene 10189
Alt Symbol ALY, ALY/REF, BEF, REF, THOC4
Species Human
Gene Type protein-coding
Description Aly/REF export factor
Other Description THO complex 4|THO complex subunit 4|ally of AML-1 and LEF-1|bZIP enhancing factor|bZIP-enhancing factor BEF|tho4|transcriptional coactivator Aly/REF
Swissprots O43672 Q86V81
Accessions EAW89699 Q86V81 AF047002 AAD09608 AF086513 BC052302 AAH52302 BU624466 EU831428 ACE87115 EU831521 ACE86440 XR_933919 NM_005782 NP_005773
Function Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX
Subcellular Location Nucleus. Nucleus speckle. Cytoplasm. Note=Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur.
Top Pathways Spliceosome, RNA transport, mRNA surveillance pathway, Herpes simplex infection

THOC4/ALY (D3R4R) Rabbit mAb - 12655 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3R4R
Western blot analysis of extracts from various cell lines using THOC4/ALY (D3R4R) Rabbit mAb.

THOC4/ALY Antibody - 11824 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

ALY (11G5) - sc-32311 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ALY (11G5): sc-32311. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

ALY (4i214) - sc-70348 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

ALY (H-154) - sc-28729 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

ALY (L-15) - sc-13719 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ALY (L-15): sc-13719. Western blot analysis of ALY expression in HeLa nuclear extract.

ALY (P-20) - sc-13720 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ALY (Q-15) - sc-30816 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ALY (Q-15): sc-30816. Western blot analysis of ALY expression in Jurkat nuclear extract.

ALY (S-17) - sc-30814 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Aly antibody - ab95962 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Aly antibody (ab95962) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg

Anti-Aly antibody - ab101979 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling Aly with ab101979 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Aly antibody - ab101981 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Aly with ab101981 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Aly antibody - ab52770 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-Aly antibody (ab52770) at 1/1000 dilutionLane 1 : a total protein extract from E coli with 50ng to 100 ng of a fusion protein of an irrelevant antigenLane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (antigen fusion protein)Lysates/proteins at 20 µg per lane.SecondaryRabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Anti-Aly antibody - ab129550 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Aly antibody (ab129550) at 1/100 dilution + A549 cell lysate at 35 µg

Anti-Aly antibody - ab136985 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Aly antibody (ab136985) at 1/500 dilution + HepG2 cell lysate at 30 µg

Anti-Aly antibody [11G5] - ab6141 from Abcam

Prices $380.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 11G5
ICC/IF image of ab6141 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6141, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ALY / THOC4 Antibody (clone 11G5) - LS-C47595 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 11G5

Anti-ALY / THOC4 Antibody - LS-C11839 from LifeSpan Bioscience

Prices $530.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal

Anti-ALY / THOC4 Antibody - LS-C126364 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ALY / THOC4 Antibody (Lys161) - LS-C193250 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ALY / THOC4 Antibody (clone 11G5) - LS-C82233 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 11G5
Immunohistochemistry showing Anti-ALY on formalin fixed paraffin embedded skin tissue

Anti-ALY / THOC4 Antibody (aa60-89, AP) - LS-C271458 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALY / THOC4 Antibody (aa60-89, APC) - LS-C271459 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALY / THOC4 Antibody (aa58-107) - LS-C31923 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
THOC4 antibody LS-C31923 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.

Anti-ALY / THOC4 Antibody (aa60-89, FITC) - LS-C271461 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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