Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling Aly with ab101979 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-Aly antibody (ab101979) at 0.1 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 5 µgLane 4 : HeLa whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of Aly by Western Blot of Immunprecipitate. ab101979, at 1 µg/ml, staining Aly in 293T whole cell lysate immunoprecipitated using ab101979 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.